Carbon 13-Metabolic Flux Analysis derived constraint-based metabolic modelling of Clostridium acetobutylicum in stressed chemostat conditions

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Carbon 13-Metabolic Flux Analysis derived constraint-based metabolic modelling of Clostridium acetobutylicum in stressed chemostat conditions. / Wallenius, Janne; Maaheimo, Hannu; Eerikäinen, Tero.

In: Bioresource Technology, Vol. 219, 01.11.2016, p. 378-386.

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@article{3b3e79d9d28d45e0b97779a46dc72c4e,
title = "Carbon 13-Metabolic Flux Analysis derived constraint-based metabolic modelling of Clostridium acetobutylicum in stressed chemostat conditions",
abstract = "The metabolism of butanol producing bacteria Clostridium acetobutylicum was studied in chemostat with glucose limited conditions, butanol stimulus, and as a reference cultivation. COnstraint-Based Reconstruction and Analysis (COBRA) was applied using additional constraints from 13C Metabolic Flux Analysis (13C-MFA) and experimental measurement results. A model consisting of 451 metabolites and 604 reactions was utilized in flux balance analysis (FBA). The stringency of the flux spaces considering different optimization objectives, i.e. growth rate maximization, ATP maintenance, and NADH/NADPH formation, for flux variance analysis (FVA) was studied in the different modelled conditions. Also a previously uncharacterized exopolysaccharide (EPS) produced by C. acetobutylicum was characterized on monosaccharide level. The major monosaccharide components of the EPS were 40 n-{\%} rhamnose, 34 n-{\%} glucose, 13 n-{\%} mannose, 10 n-{\%} galactose, and 2 n-{\%} arabinose. The EPS was studied to have butanol adsorbing property, 70 (butanol) mg (EPS) g−1 at 37 °C.",
keywords = "C Metabolic Flux Analysis, Butanol stress, C. acetobutylicum, COBRA, Exopolysaccharide",
author = "Janne Wallenius and Hannu Maaheimo and Tero Eerik{\"a}inen",
year = "2016",
month = "11",
day = "1",
doi = "10.1016/j.biortech.2016.07.137",
language = "English",
volume = "219",
pages = "378--386",
journal = "Bioresource Technology",
issn = "0960-8524",

}

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TY - JOUR

T1 - Carbon 13-Metabolic Flux Analysis derived constraint-based metabolic modelling of Clostridium acetobutylicum in stressed chemostat conditions

AU - Wallenius, Janne

AU - Maaheimo, Hannu

AU - Eerikäinen, Tero

PY - 2016/11/1

Y1 - 2016/11/1

N2 - The metabolism of butanol producing bacteria Clostridium acetobutylicum was studied in chemostat with glucose limited conditions, butanol stimulus, and as a reference cultivation. COnstraint-Based Reconstruction and Analysis (COBRA) was applied using additional constraints from 13C Metabolic Flux Analysis (13C-MFA) and experimental measurement results. A model consisting of 451 metabolites and 604 reactions was utilized in flux balance analysis (FBA). The stringency of the flux spaces considering different optimization objectives, i.e. growth rate maximization, ATP maintenance, and NADH/NADPH formation, for flux variance analysis (FVA) was studied in the different modelled conditions. Also a previously uncharacterized exopolysaccharide (EPS) produced by C. acetobutylicum was characterized on monosaccharide level. The major monosaccharide components of the EPS were 40 n-% rhamnose, 34 n-% glucose, 13 n-% mannose, 10 n-% galactose, and 2 n-% arabinose. The EPS was studied to have butanol adsorbing property, 70 (butanol) mg (EPS) g−1 at 37 °C.

AB - The metabolism of butanol producing bacteria Clostridium acetobutylicum was studied in chemostat with glucose limited conditions, butanol stimulus, and as a reference cultivation. COnstraint-Based Reconstruction and Analysis (COBRA) was applied using additional constraints from 13C Metabolic Flux Analysis (13C-MFA) and experimental measurement results. A model consisting of 451 metabolites and 604 reactions was utilized in flux balance analysis (FBA). The stringency of the flux spaces considering different optimization objectives, i.e. growth rate maximization, ATP maintenance, and NADH/NADPH formation, for flux variance analysis (FVA) was studied in the different modelled conditions. Also a previously uncharacterized exopolysaccharide (EPS) produced by C. acetobutylicum was characterized on monosaccharide level. The major monosaccharide components of the EPS were 40 n-% rhamnose, 34 n-% glucose, 13 n-% mannose, 10 n-% galactose, and 2 n-% arabinose. The EPS was studied to have butanol adsorbing property, 70 (butanol) mg (EPS) g−1 at 37 °C.

KW - C Metabolic Flux Analysis

KW - Butanol stress

KW - C. acetobutylicum

KW - COBRA

KW - Exopolysaccharide

UR - http://www.scopus.com/inward/record.url?scp=84980426468&partnerID=8YFLogxK

U2 - 10.1016/j.biortech.2016.07.137

DO - 10.1016/j.biortech.2016.07.137

M3 - Article

VL - 219

SP - 378

EP - 386

JO - Bioresource Technology

JF - Bioresource Technology

SN - 0960-8524

ER -

ID: 6883193