Capillary electrophoresis for the monitoring of phenolic compounds in bioprocesses

Heidi Turkia*, Heli Siren, Merja Penttila, Juha-Pekka Pitkanen

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

Abstract

Hydrolysates of lignocellulosic biomass, used as substrates for the sustainable production of fuels and chemicals often contain high amounts of phenolic compounds inhibiting the production microbiota. Quantification of these inhibitor compounds may help to understand possible difficulties in bioprocessing and further the development of more efficient, robust and tolerable processes. A separation method based on capillary electrophoresis with UV detection was developed for the simultaneous quantification of 10 phenolic compounds that may have inhibitor properties. Intraday relative standard deviations were less than 0.7% for migration times and between 2.6% and 6.4% for peak areas. Interday relative standard deviations were less than 3.0% for migration times and between 5.0% and 7.2% for peak areas. The method was applied to demonstrate that Saccharomyces cerevisiae was able to decrease the concentrations of vanillin, coniferyl aldehyde, syringaldehyde, acetoguaiacone and cinnamic acid during the cultivation, whereas the concentrations of phenols increased. (c) 2013 Elsevier B.V. All rights reserved.

Original languageEnglish
Pages (from-to)175-180
Number of pages6
JournalJOURNAL OF CHROMATOGRAPHY A
Volume1278
DOIs
Publication statusPublished - 22 Feb 2013
MoE publication typeA1 Journal article-refereed

Keywords

  • Bioprocess monitoring
  • Capillary electrophoresis
  • Phenolic compounds
  • IONIZATION-MASS-SPECTROMETRY
  • DEGRADATION-PRODUCTS
  • SACCHAROMYCES-CEREVISIAE
  • FERMENTATION INHIBITORS
  • ZONE-ELECTROPHORESIS
  • FUEL ETHANOL
  • AGED PAPERS
  • ACIDS
  • LIGNOCELLULOSE
  • BIOMASS

Cite this