Brachybacterium sp. CH-KOV3 isolated from an oil-polluted environment–a new producer of levan

Research output: Contribution to journalArticleScientificpeer-review

Researchers

  • Aleksandra Djurić
  • Gordana Gojgić-Cvijović
  • Dragica Jakovljević
  • Branka Kekez
  • Jovana Stefanović Kojić
  • Maija Liisa Mattinen
  • Inka Elina Harju
  • Miroslav M. Vrvić
  • Vladimir P. Beškoski

Research units

  • University of Belgrade
  • University of Turku

Abstract

Various microorganisms isolated from polluted environments, such as Pseudomonas sp. and Micrococcus sp. can synthesize exopolysaccharides (EPSs) which are natural, non-toxic and biodegradable polymers. EPSs play a key role in protection of microbial cells under various external influences. For humans, these substances have potential use in many industries. EPSs can be applied as a flavor or a fragrance carrier, an emulsifier, a stabilizer, a prebiotic, an antioxidant or an antitumor agent. In this study, we characterized an environmental microorganism that produces EPS, optimized EPS production by this strain and characterized the EPS produced. Isolate CH-KOV3 was identified as Brachybacterium paraconglomeratum. The sucrose level in the growth medium greatly influenced EPS production, and the highest yield was when the microorganism was incubated in media with 500 g/L of sucrose. The optimal temperature and pH were 28 °C and 7.0, respectively. The nuclear magnetic resonance (NMR) results and GC–MS analysis confirmed that the residues were D-fructofuranosyl residues with β-configuration, where fructose units are linked by β-2,6-glycosidic bonds, with β-2,1-linked branches. All these data indicate that the investigated EPS is a levan-type polysaccharide. Thus, it was concluded that Brachybacterium sp. CH-KOV3 could constitute a new source for production of the bioactive polysaccharide, levan.

Details

Original languageEnglish
Pages (from-to)311-321
Number of pages11
JournalInternational Journal of Biological Macromolecules
Volume104
Publication statusPublished - 1 Nov 2017
MoE publication typeA1 Journal article-refereed

    Research areas

  • Brachybacterium paraconglomeratum, Exopolysaccharides, Polluted environments

ID: 14387221