Boar spermatozoa as a biosensor for detecting toxic substances in indoor dust and aerosols

M. A. Andersson, R. Mikkola, S. Rasimus, D. Hoornstra, P. Salin, R. Rahkila, M. Heikkinen, S. Mattila, J. Peltola, S. Kalso, M. Salkinoja-Salonen*

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

Abstract

The presence, quantity and origins of potentially toxic airborne substances were searched in moisture damaged indoor environments, where building related ill health symptoms were suspected and reference sites with no health complaints. Boar spermatozoa were used as the toxicity sensor. Indoor aerosols and dusts were collected from kindergartens, schools, offices and residences (n = 25) by electrostatic filtering, vacuuming, wiping from elevated surfaces and from the interior of personal computers. Toxicity was measured from the ethanol or methanol extracts of the dusts and aerosols. EC(50) was expressed as the lowest concentration of the airborne substance that inhibited motility of >50% of the exposed sperm cells compared to vehicle control, within 30 min, 1 day or 3-4 days of exposure. Remarkably toxic aerosols (EC(50)

Original languageEnglish
Pages (from-to)2041-2052
Number of pages12
JournalTOXICOLOGY IN VITRO
Volume24
Issue number7
DOIs
Publication statusPublished - Oct 2010
MoE publication typeA1 Journal article-refereed

Keywords

  • Aerosol toxicity
  • Dust
  • Boar sperm
  • Amylosin
  • Cereulide
  • Stephacidin B
  • Valinomycin
  • CEREUS EMETIC TOXIN
  • FOOD-CONTACT PAPER
  • BACILLUS-CEREUS
  • STEPHACIDIN-B
  • ACREMONIUM-EXUVIARUM
  • BUILDING-MATERIALS
  • DAMAGED BUILDINGS
  • MAMMALIAN-CELLS
  • STRAINS
  • VALINOMYCIN

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