Bioreducible Hydrophobin-Stabilized Supraparticles for Selective Intracellular Release

Research output: Contribution to journalArticleScientificpeer-review

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Bioreducible Hydrophobin-Stabilized Supraparticles for Selective Intracellular Release. / Maiolo, Daniele; Pigliacelli, Claudia; Sánchez Moreno, Paola; Violatto, Martina Bruna; Talamini, Laura; Tirotta, Ilaria; Piccirillo, Rosanna; Zucchetti, Massimo; Morosi, Lavinia; Frapolli, Roberta; Candiani, Gabriele; Bigini, Paolo; Metrangolo, Pierangelo; Baldelli Bombelli, Francesca.

In: ACS Nano, Vol. 2017, 14.08.2017, p. 9413-9423.

Research output: Contribution to journalArticleScientificpeer-review

Harvard

Maiolo, D, Pigliacelli, C, Sánchez Moreno, P, Violatto, MB, Talamini, L, Tirotta, I, Piccirillo, R, Zucchetti, M, Morosi, L, Frapolli, R, Candiani, G, Bigini, P, Metrangolo, P & Baldelli Bombelli, F 2017, 'Bioreducible Hydrophobin-Stabilized Supraparticles for Selective Intracellular Release', ACS Nano, vol. 2017, pp. 9413-9423. https://doi.org/10.1021/acsnano.7b04979

APA

Maiolo, D., Pigliacelli, C., Sánchez Moreno, P., Violatto, M. B., Talamini, L., Tirotta, I., ... Baldelli Bombelli, F. (2017). Bioreducible Hydrophobin-Stabilized Supraparticles for Selective Intracellular Release. ACS Nano, 2017, 9413-9423. https://doi.org/10.1021/acsnano.7b04979

Vancouver

Maiolo D, Pigliacelli C, Sánchez Moreno P, Violatto MB, Talamini L, Tirotta I et al. Bioreducible Hydrophobin-Stabilized Supraparticles for Selective Intracellular Release. ACS Nano. 2017 Aug 14;2017:9413-9423. https://doi.org/10.1021/acsnano.7b04979

Author

Maiolo, Daniele ; Pigliacelli, Claudia ; Sánchez Moreno, Paola ; Violatto, Martina Bruna ; Talamini, Laura ; Tirotta, Ilaria ; Piccirillo, Rosanna ; Zucchetti, Massimo ; Morosi, Lavinia ; Frapolli, Roberta ; Candiani, Gabriele ; Bigini, Paolo ; Metrangolo, Pierangelo ; Baldelli Bombelli, Francesca. / Bioreducible Hydrophobin-Stabilized Supraparticles for Selective Intracellular Release. In: ACS Nano. 2017 ; Vol. 2017. pp. 9413-9423.

Bibtex - Download

@article{075958033a0846b58346d7908504dde9,
title = "Bioreducible Hydrophobin-Stabilized Supraparticles for Selective Intracellular Release",
abstract = "One of the main hurdles in nanomedicine is the low stability of drug–nanocarrier complexes as well as the drug delivery efficiency in the region-of-interest. Here, we describe the use of the film-forming protein hydrophobin HFBII to organize dodecanethiol-protected gold nanoparticles (NPs) into well-defined supraparticles (SPs). The obtained SPs are exceptionally stable in vivo and efficiently encapsulate hydrophobic drug molecules. The HFBII film prevents massive release of the encapsulated drug, which, instead, is activated by selective SP disassembly triggered intracellularly by glutathione reduction of the protein film. As a consequence, the therapeutic efficiency of an encapsulated anticancer drug is highly enhanced (2 orders of magnitude decrease in IC50). Biodistribution and pharmacokinetics studies demonstrate the high stability of the loaded SPs in the bloodstream and the selective release of the payloads once taken up in the tissues. Overall, our results provide a rationale for the development of bioreducible and multifunctional nanomedicines.",
keywords = "drug release, gold nanoparticle, hydrophobin, nanobio interface, self-assembly, supraparticle",
author = "Daniele Maiolo and Claudia Pigliacelli and {S{\'a}nchez Moreno}, Paola and Violatto, {Martina Bruna} and Laura Talamini and Ilaria Tirotta and Rosanna Piccirillo and Massimo Zucchetti and Lavinia Morosi and Roberta Frapolli and Gabriele Candiani and Paolo Bigini and Pierangelo Metrangolo and {Baldelli Bombelli}, Francesca",
note = "doi: 10.1021/acsnano.7b04979",
year = "2017",
month = "8",
day = "14",
doi = "10.1021/acsnano.7b04979",
language = "English",
volume = "2017",
pages = "9413--9423",
journal = "ACS Nano",
issn = "1936-0851",
publisher = "AMERICAN CHEMICAL SOCIETY",

}

RIS - Download

TY - JOUR

T1 - Bioreducible Hydrophobin-Stabilized Supraparticles for Selective Intracellular Release

AU - Maiolo, Daniele

AU - Pigliacelli, Claudia

AU - Sánchez Moreno, Paola

AU - Violatto, Martina Bruna

AU - Talamini, Laura

AU - Tirotta, Ilaria

AU - Piccirillo, Rosanna

AU - Zucchetti, Massimo

AU - Morosi, Lavinia

AU - Frapolli, Roberta

AU - Candiani, Gabriele

AU - Bigini, Paolo

AU - Metrangolo, Pierangelo

AU - Baldelli Bombelli, Francesca

N1 - doi: 10.1021/acsnano.7b04979

PY - 2017/8/14

Y1 - 2017/8/14

N2 - One of the main hurdles in nanomedicine is the low stability of drug–nanocarrier complexes as well as the drug delivery efficiency in the region-of-interest. Here, we describe the use of the film-forming protein hydrophobin HFBII to organize dodecanethiol-protected gold nanoparticles (NPs) into well-defined supraparticles (SPs). The obtained SPs are exceptionally stable in vivo and efficiently encapsulate hydrophobic drug molecules. The HFBII film prevents massive release of the encapsulated drug, which, instead, is activated by selective SP disassembly triggered intracellularly by glutathione reduction of the protein film. As a consequence, the therapeutic efficiency of an encapsulated anticancer drug is highly enhanced (2 orders of magnitude decrease in IC50). Biodistribution and pharmacokinetics studies demonstrate the high stability of the loaded SPs in the bloodstream and the selective release of the payloads once taken up in the tissues. Overall, our results provide a rationale for the development of bioreducible and multifunctional nanomedicines.

AB - One of the main hurdles in nanomedicine is the low stability of drug–nanocarrier complexes as well as the drug delivery efficiency in the region-of-interest. Here, we describe the use of the film-forming protein hydrophobin HFBII to organize dodecanethiol-protected gold nanoparticles (NPs) into well-defined supraparticles (SPs). The obtained SPs are exceptionally stable in vivo and efficiently encapsulate hydrophobic drug molecules. The HFBII film prevents massive release of the encapsulated drug, which, instead, is activated by selective SP disassembly triggered intracellularly by glutathione reduction of the protein film. As a consequence, the therapeutic efficiency of an encapsulated anticancer drug is highly enhanced (2 orders of magnitude decrease in IC50). Biodistribution and pharmacokinetics studies demonstrate the high stability of the loaded SPs in the bloodstream and the selective release of the payloads once taken up in the tissues. Overall, our results provide a rationale for the development of bioreducible and multifunctional nanomedicines.

KW - drug release

KW - gold nanoparticle

KW - hydrophobin

KW - nanobio interface

KW - self-assembly

KW - supraparticle

U2 - 10.1021/acsnano.7b04979

DO - 10.1021/acsnano.7b04979

M3 - Article

VL - 2017

SP - 9413

EP - 9423

JO - ACS Nano

JF - ACS Nano

SN - 1936-0851

ER -

ID: 14856393