Biopolymer-Capped Pyrazinamide-Loaded Colloidosomes : In Vitro Characterization and Bioavailability Studies

Avi Singh; Sabya Sachi Das; Janne Ruokolainen; Kavindra Kumar Kesari; Sandeep Kumar Singh

doi:10.1021/acsomega.3c03135

Biopolymer-Capped Pyrazinamide-Loaded Colloidosomes : In Vitro Characterization and Bioavailability Studies

Avi Singh
, Sabya Sachi Das
, Janne Ruokolainen
, Kavindra Kumar Kesari^*
, Sandeep Kumar Singh^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Scientific › peer-review

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Abstract

This study aimed to prepare colloidosome particles loaded with pyrazinamide (PZA). These drug-loaded colloidosomes were prepared using an in situ gelation technique using a central composite design with a shell made of calcium carbonate (CaCO₃) particles. Optimal amounts of 150 mg of CaCO₃, sodium alginate (2%), and 400 mg of poly(3-hydroxybutyrate-co-3-hydroxy valerate) (PHBV) concentration resulted in the maximum drug loading and efficient release profile. Field emission scanning electron microscopy results showed spherical porous particles with a good coating of the PHBV polymer. Additionally, Fourier transform infrared (FTIR) spectroscopy, differential scanning calorimetry (DSC), thermogravimetric and differential thermal analysis (TGA-DTA), and X-ray diffraction (XRD) analysis showed good compatibility between the drug and excipients. The pharmacokinetic studies demonstrated that the drug-loaded colloidosomes resulted in 4.26 times higher plasma drug concentrations with C_max values of 32.386 ± 2.744 mcg/mL (PZA solution) and 115.868 ± 53.581 mcg/mL (PZA-loaded colloidosomes) and AUC_0-t values of 61.24 mcg-h/mL (PZA solution) and 260.9 mcg-h/mL (PZA-loaded colloidosomes), indicating that colloidosomes have the potential to be effective drug carriers for delivering PZA to the target site.

Original language	English
Pages (from-to)	25515–25524
Journal	ACS Omega
Volume	8
Issue number	28
DOIs	https://doi.org/10.1021/acsomega.3c03135
Publication status	Published - 6 Jul 2023
MoE publication type	A1 Journal article-refereed

Funding

A.S. acknowledges the financial assistance provided by the Department of Science and Technology, Government of India, under Inspire Programme (IF: 180432). The authors acknowledge the central instrument facility of the Birla Institute of Technology. The graphical abstract has been created using Biorender.

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SCI_Singh_etal_ACS_Omega_2023Final published version, 5.39 MBLicence: CC BY

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title = "Biopolymer-Capped Pyrazinamide-Loaded Colloidosomes : In Vitro Characterization and Bioavailability Studies",

abstract = "This study aimed to prepare colloidosome particles loaded with pyrazinamide (PZA). These drug-loaded colloidosomes were prepared using an in situ gelation technique using a central composite design with a shell made of calcium carbonate (CaCO3) particles. Optimal amounts of 150 mg of CaCO3, sodium alginate (2\%), and 400 mg of poly(3-hydroxybutyrate-co-3-hydroxy valerate) (PHBV) concentration resulted in the maximum drug loading and efficient release profile. Field emission scanning electron microscopy results showed spherical porous particles with a good coating of the PHBV polymer. Additionally, Fourier transform infrared (FTIR) spectroscopy, differential scanning calorimetry (DSC), thermogravimetric and differential thermal analysis (TGA-DTA), and X-ray diffraction (XRD) analysis showed good compatibility between the drug and excipients. The pharmacokinetic studies demonstrated that the drug-loaded colloidosomes resulted in 4.26 times higher plasma drug concentrations with Cmax values of 32.386 ± 2.744 mcg/mL (PZA solution) and 115.868 ± 53.581 mcg/mL (PZA-loaded colloidosomes) and AUC0-t values of 61.24 mcg-h/mL (PZA solution) and 260.9 mcg-h/mL (PZA-loaded colloidosomes), indicating that colloidosomes have the potential to be effective drug carriers for delivering PZA to the target site.",

author = "Avi Singh and Das, \{Sabya Sachi\} and Janne Ruokolainen and Kesari, \{Kavindra Kumar\} and Singh, \{Sandeep Kumar\}",

note = "Funding Information: A.S. acknowledges the financial assistance provided by the Department of Science and Technology, Government of India, under Inspire Programme (IF: 180432). The authors acknowledge the central instrument facility of the Birla Institute of Technology. The graphical abstract has been created using Biorender. Publisher Copyright: {\textcopyright} 2023 The Authors. Published by American Chemical Society.",

year = "2023",

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doi = "10.1021/acsomega.3c03135",

language = "English",

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AU - Singh, Avi

AU - Das, Sabya Sachi

AU - Ruokolainen, Janne

AU - Kesari, Kavindra Kumar

AU - Singh, Sandeep Kumar

N1 - Funding Information: A.S. acknowledges the financial assistance provided by the Department of Science and Technology, Government of India, under Inspire Programme (IF: 180432). The authors acknowledge the central instrument facility of the Birla Institute of Technology. The graphical abstract has been created using Biorender. Publisher Copyright: © 2023 The Authors. Published by American Chemical Society.

PY - 2023/7/6

Y1 - 2023/7/6

N2 - This study aimed to prepare colloidosome particles loaded with pyrazinamide (PZA). These drug-loaded colloidosomes were prepared using an in situ gelation technique using a central composite design with a shell made of calcium carbonate (CaCO3) particles. Optimal amounts of 150 mg of CaCO3, sodium alginate (2%), and 400 mg of poly(3-hydroxybutyrate-co-3-hydroxy valerate) (PHBV) concentration resulted in the maximum drug loading and efficient release profile. Field emission scanning electron microscopy results showed spherical porous particles with a good coating of the PHBV polymer. Additionally, Fourier transform infrared (FTIR) spectroscopy, differential scanning calorimetry (DSC), thermogravimetric and differential thermal analysis (TGA-DTA), and X-ray diffraction (XRD) analysis showed good compatibility between the drug and excipients. The pharmacokinetic studies demonstrated that the drug-loaded colloidosomes resulted in 4.26 times higher plasma drug concentrations with Cmax values of 32.386 ± 2.744 mcg/mL (PZA solution) and 115.868 ± 53.581 mcg/mL (PZA-loaded colloidosomes) and AUC0-t values of 61.24 mcg-h/mL (PZA solution) and 260.9 mcg-h/mL (PZA-loaded colloidosomes), indicating that colloidosomes have the potential to be effective drug carriers for delivering PZA to the target site.

AB - This study aimed to prepare colloidosome particles loaded with pyrazinamide (PZA). These drug-loaded colloidosomes were prepared using an in situ gelation technique using a central composite design with a shell made of calcium carbonate (CaCO3) particles. Optimal amounts of 150 mg of CaCO3, sodium alginate (2%), and 400 mg of poly(3-hydroxybutyrate-co-3-hydroxy valerate) (PHBV) concentration resulted in the maximum drug loading and efficient release profile. Field emission scanning electron microscopy results showed spherical porous particles with a good coating of the PHBV polymer. Additionally, Fourier transform infrared (FTIR) spectroscopy, differential scanning calorimetry (DSC), thermogravimetric and differential thermal analysis (TGA-DTA), and X-ray diffraction (XRD) analysis showed good compatibility between the drug and excipients. The pharmacokinetic studies demonstrated that the drug-loaded colloidosomes resulted in 4.26 times higher plasma drug concentrations with Cmax values of 32.386 ± 2.744 mcg/mL (PZA solution) and 115.868 ± 53.581 mcg/mL (PZA-loaded colloidosomes) and AUC0-t values of 61.24 mcg-h/mL (PZA solution) and 260.9 mcg-h/mL (PZA-loaded colloidosomes), indicating that colloidosomes have the potential to be effective drug carriers for delivering PZA to the target site.

UR - https://www.scopus.com/pages/publications/85164907637

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DO - 10.1021/acsomega.3c03135

M3 - Article

AN - SCOPUS:85164907637

SN - 2470-1343

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EP - 25524

JO - ACS Omega

JF - ACS Omega

IS - 28

ER -

Biopolymer-Capped Pyrazinamide-Loaded Colloidosomes : In Vitro Characterization and Bioavailability Studies

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