Biomimetic Photocurable Three-Dimensional Printed Nerve Guidance Channels with Aligned Cryomatrix Lumen for Peripheral Nerve Regeneration

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Biomimetic Photocurable Three-Dimensional Printed Nerve Guidance Channels with Aligned Cryomatrix Lumen for Peripheral Nerve Regeneration. / Singh, Anamika; Asikainen, Sanja; Teotia, Arun K.; Shiekh, Parvaiz A.; Huotilainen, Eero; Qayoom, Irfan; Partanen, Jouni; Seppälä, Jukka; Kumar, Ashok.

In: ACS Applied Materials and Interfaces, Vol. 10, No. 50, 19.12.2018, p. 43327-43342.

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@article{66aef69a7de94c74b3f7ddc586b7f851,
title = "Biomimetic Photocurable Three-Dimensional Printed Nerve Guidance Channels with Aligned Cryomatrix Lumen for Peripheral Nerve Regeneration",
abstract = "Repair and regeneration of critically injured peripheral nerves is one of the most challenging reconstructive surgeries. Currently available and FDA approved nerve guidance channels (NGCs) are suitable for small gap injuries, and their biological performance is inferior to that of autografts. Development of biomimetic NGCs with clinically relevant geometrical and biological characteristics such as topographical, biochemical, and haptotactic cues could offer better regeneration of the long-gap complex nerve injuries. Here, in this study, we present the development and preclinical analysis of three-dimensional (3D) printed aligned cryomatrix-filled NGCs along with nerve growth factor (NGF) (aCG + NGF) for peripheral nerve regeneration. We demonstrated the application of these aCG + NGF NGCs in the enhanced and successful regeneration of a critically injured rat sciatic nerve in comparison to random cryogel-filled NGCs, multichannel and clinically preferred hollow conduits, and the gold standard autografts. Our results indicated similar effect of the aCG + NGF NGCs viz-a-viz that of the autografts, and they not only enhanced the overall regenerated nerve physiology but could also mimic the cellular aspects of regeneration. This study emphasizes the paradigm that these biomimetic 3D printed NGCs will lead to a better functional regenerative outcome under clinical settings.",
keywords = "3D printing, aligned cryogel, guidance channel, nerve regeneration, stereolithography",
author = "Anamika Singh and Sanja Asikainen and Teotia, {Arun K.} and Shiekh, {Parvaiz A.} and Eero Huotilainen and Irfan Qayoom and Jouni Partanen and Jukka Sepp{\"a}l{\"a} and Ashok Kumar",
year = "2018",
month = "12",
day = "19",
doi = "10.1021/acsami.8b11677",
language = "English",
volume = "10",
pages = "43327--43342",
journal = "ACS Applied Materials and Interfaces",
issn = "1944-8244",
publisher = "AMERICAN CHEMICAL SOCIETY",
number = "50",

}

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TY - JOUR

T1 - Biomimetic Photocurable Three-Dimensional Printed Nerve Guidance Channels with Aligned Cryomatrix Lumen for Peripheral Nerve Regeneration

AU - Singh, Anamika

AU - Asikainen, Sanja

AU - Teotia, Arun K.

AU - Shiekh, Parvaiz A.

AU - Huotilainen, Eero

AU - Qayoom, Irfan

AU - Partanen, Jouni

AU - Seppälä, Jukka

AU - Kumar, Ashok

PY - 2018/12/19

Y1 - 2018/12/19

N2 - Repair and regeneration of critically injured peripheral nerves is one of the most challenging reconstructive surgeries. Currently available and FDA approved nerve guidance channels (NGCs) are suitable for small gap injuries, and their biological performance is inferior to that of autografts. Development of biomimetic NGCs with clinically relevant geometrical and biological characteristics such as topographical, biochemical, and haptotactic cues could offer better regeneration of the long-gap complex nerve injuries. Here, in this study, we present the development and preclinical analysis of three-dimensional (3D) printed aligned cryomatrix-filled NGCs along with nerve growth factor (NGF) (aCG + NGF) for peripheral nerve regeneration. We demonstrated the application of these aCG + NGF NGCs in the enhanced and successful regeneration of a critically injured rat sciatic nerve in comparison to random cryogel-filled NGCs, multichannel and clinically preferred hollow conduits, and the gold standard autografts. Our results indicated similar effect of the aCG + NGF NGCs viz-a-viz that of the autografts, and they not only enhanced the overall regenerated nerve physiology but could also mimic the cellular aspects of regeneration. This study emphasizes the paradigm that these biomimetic 3D printed NGCs will lead to a better functional regenerative outcome under clinical settings.

AB - Repair and regeneration of critically injured peripheral nerves is one of the most challenging reconstructive surgeries. Currently available and FDA approved nerve guidance channels (NGCs) are suitable for small gap injuries, and their biological performance is inferior to that of autografts. Development of biomimetic NGCs with clinically relevant geometrical and biological characteristics such as topographical, biochemical, and haptotactic cues could offer better regeneration of the long-gap complex nerve injuries. Here, in this study, we present the development and preclinical analysis of three-dimensional (3D) printed aligned cryomatrix-filled NGCs along with nerve growth factor (NGF) (aCG + NGF) for peripheral nerve regeneration. We demonstrated the application of these aCG + NGF NGCs in the enhanced and successful regeneration of a critically injured rat sciatic nerve in comparison to random cryogel-filled NGCs, multichannel and clinically preferred hollow conduits, and the gold standard autografts. Our results indicated similar effect of the aCG + NGF NGCs viz-a-viz that of the autografts, and they not only enhanced the overall regenerated nerve physiology but could also mimic the cellular aspects of regeneration. This study emphasizes the paradigm that these biomimetic 3D printed NGCs will lead to a better functional regenerative outcome under clinical settings.

KW - 3D printing

KW - aligned cryogel

KW - guidance channel

KW - nerve regeneration

KW - stereolithography

UR - http://www.scopus.com/inward/record.url?scp=85058666518&partnerID=8YFLogxK

U2 - 10.1021/acsami.8b11677

DO - 10.1021/acsami.8b11677

M3 - Article

VL - 10

SP - 43327

EP - 43342

JO - ACS Applied Materials and Interfaces

JF - ACS Applied Materials and Interfaces

SN - 1944-8244

IS - 50

ER -

ID: 30784124