TY - JOUR
T1 - Biological and technical study of a partial-SHARON reactor at laboratory scale
T2 - Effect of hydraulic retention time
AU - González-Martínez, A.
AU - Calderón, K.
AU - Albuquerque, A.
AU - Hontoria, E.
AU - González-López, J.
AU - Guisado, I. M.
AU - Osorio, F.
PY - 2013/2
Y1 - 2013/2
N2 - This study was on the technical and biological characteristics of a partial-SHARON submerged-filter bioreactor of 3 L. The main focus was the influence of the hydraulic retention time (HRT) on biofilms. For this purpose, we used molecular tools based on the partial 16S rRNA genes. The results showed that the HRT may affect the nitrification processes of a bioreactor using synthetic wastewater containing 600 mg/L of ammonia. It was found that an HRT of 0.5 day transformed 100 % of the ammonium into nitrite. However, when the HRT was decreased to 0.4 day, there was a significant reduction (35 %) in the quantity of ammonia transformed, which confirmed the complexity of the system operation. Moreover, a PCR-TGGE approach highlighted the differences observed. The results obtained showed that an HRT of 0.5 day reduced bacterial biodiversity in the biofilms, which were mainly formed by Nitrosomonas and Diaphorobacter. In contrast, an HRT of 0.4 day facilitated the formation of heterogeneous biofilms formed by nitrifying bacteria, such as Nitrosomonas sp., Nitrosospira sp., and Nitrosovibrio sp.).
AB - This study was on the technical and biological characteristics of a partial-SHARON submerged-filter bioreactor of 3 L. The main focus was the influence of the hydraulic retention time (HRT) on biofilms. For this purpose, we used molecular tools based on the partial 16S rRNA genes. The results showed that the HRT may affect the nitrification processes of a bioreactor using synthetic wastewater containing 600 mg/L of ammonia. It was found that an HRT of 0.5 day transformed 100 % of the ammonium into nitrite. However, when the HRT was decreased to 0.4 day, there was a significant reduction (35 %) in the quantity of ammonia transformed, which confirmed the complexity of the system operation. Moreover, a PCR-TGGE approach highlighted the differences observed. The results obtained showed that an HRT of 0.5 day reduced bacterial biodiversity in the biofilms, which were mainly formed by Nitrosomonas and Diaphorobacter. In contrast, an HRT of 0.4 day facilitated the formation of heterogeneous biofilms formed by nitrifying bacteria, such as Nitrosomonas sp., Nitrosospira sp., and Nitrosovibrio sp.).
KW - Hydraulic retention time (HRT)
KW - Nitrogen removal
KW - Partial nitrification
KW - SHARON process
KW - Submerged biofilter
KW - Wastewater treatment
UR - http://www.scopus.com/inward/record.url?scp=84879716097&partnerID=8YFLogxK
U2 - 10.1007/s00449-012-0772-7
DO - 10.1007/s00449-012-0772-7
M3 - Article
C2 - 22767397
AN - SCOPUS:84879716097
VL - 36
SP - 173
EP - 184
JO - BIOPROCESS AND BIOSYSTEMS ENGINEERING
JF - BIOPROCESS AND BIOSYSTEMS ENGINEERING
SN - 1615-7591
IS - 2
ER -