Biological activity of multicomponent bio-hydrogels loaded with tragacanth gum

Roberta Teixeira Polez; Maria Morits; Christopher Jonkergouw; Josphat Phiri; Juan José Valle-Delgado; Jukka Seppälä; Markus B. Linder; Thaddeus Maloney; Orlando Rojas Gaona; Monika Österberg

doi:10.1016/j.ijbiomac.2022.06.153

Biological activity of multicomponent bio-hydrogels loaded with tragacanth gum

Roberta Teixeira Polez, Maria Morits, Christopher Jonkergouw, Josphat Phiri, Juan José Valle-Delgado, Jukka Seppälä, Markus B. Linder, Thaddeus Maloney, Orlando Rojas Gaona, Monika Österberg^*

^*Corresponding author for this work

University of British Columbia

Research output: Contribution to journal › Article › Scientific › peer-review

31 Citations (Scopus)

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Abstract

Producing hydrogels capable of mimicking the biomechanics of soft tissue remains a challenge. We explore the potential of plant-based hydrogels as polysaccharide tragacanth gum and antioxidant lignin nanoparticles in bioactive multicomponent hydrogels for tissue engineering. These natural components are combined with TEMPO-oxidized cellulose nanofibrils, a material with known shear thinning behavior. Hydrogels presented tragacanth gum (TG) concentration-dependent rheological properties suitable for extrusion 3D printing. TG enhanced the swelling capacity up to 645% and the degradation rate up to 1.3%/day for hydrogels containing 75% of TG. Young's moduli of the hydrogels varied from 5.0 to 11.6 kPa and were comparable to soft tissues like skin and muscle. In vitro cell viability assays revealed that the scaffolds were non-toxic and promoted proliferation of hepatocellular carcinoma HepG2 cells. Therefore, the plant-based hydrogels designed in this work have a significant potential for tissue engineering.

Original language	English
Pages (from-to)	691-704
Number of pages	14
Journal	International Journal of Biological Macromolecules
Volume	215
Early online date	4 Jul 2022
DOIs	https://doi.org/10.1016/j.ijbiomac.2022.06.153
Publication status	Published - 31 Aug 2022
MoE publication type	A1 Journal article-refereed

Keywords

Tragacanth gum
Hydrogels
Cellulose nanofibrils
Lignin nanoparticles (LNP)
Bioprinting
Tissue engineering

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.ijbiomac.2022.06.153Licence: CC BY

CHEM_Teixeira-Polez_et_al_Biological_activity_2022_Int_J_Biological_MacromoleculesFinal published version, 8.79 MBLicence: CC BY

Cite this

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title = "Biological activity of multicomponent bio-hydrogels loaded with tragacanth gum",

abstract = "Producing hydrogels capable of mimicking the biomechanics of soft tissue remains a challenge. We explore the potential of plant-based hydrogels as polysaccharide tragacanth gum and antioxidant lignin nanoparticles in bioactive multicomponent hydrogels for tissue engineering. These natural components are combined with TEMPO-oxidized cellulose nanofibrils, a material with known shear thinning behavior. Hydrogels presented tragacanth gum (TG) concentration-dependent rheological properties suitable for extrusion 3D printing. TG enhanced the swelling capacity up to 645% and the degradation rate up to 1.3%/day for hydrogels containing 75% of TG. Young's moduli of the hydrogels varied from 5.0 to 11.6 kPa and were comparable to soft tissues like skin and muscle. In vitro cell viability assays revealed that the scaffolds were non-toxic and promoted proliferation of hepatocellular carcinoma HepG2 cells. Therefore, the plant-based hydrogels designed in this work have a significant potential for tissue engineering.",

keywords = "Tragacanth gum, Hydrogels, Cellulose nanofibrils, Lignin nanoparticles (LNP), Bioprinting, Tissue engineering",

author = "{Teixeira Polez}, Roberta and Maria Morits and Christopher Jonkergouw and Josphat Phiri and Valle-Delgado, {Juan Jos{\'e}} and Jukka Sepp{\"a}l{\"a} and Linder, {Markus B.} and Thaddeus Maloney and {Rojas Gaona}, Orlando and Monika {\"O}sterberg",

year = "2022",

month = aug,

day = "31",

doi = "10.1016/j.ijbiomac.2022.06.153",

language = "English",

volume = "215",

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AU - Teixeira Polez, Roberta

AU - Morits, Maria

AU - Jonkergouw, Christopher

AU - Phiri, Josphat

AU - Valle-Delgado, Juan José

AU - Seppälä, Jukka

AU - Linder, Markus B.

AU - Maloney, Thaddeus

AU - Rojas Gaona, Orlando

AU - Österberg, Monika

PY - 2022/8/31

Y1 - 2022/8/31

N2 - Producing hydrogels capable of mimicking the biomechanics of soft tissue remains a challenge. We explore the potential of plant-based hydrogels as polysaccharide tragacanth gum and antioxidant lignin nanoparticles in bioactive multicomponent hydrogels for tissue engineering. These natural components are combined with TEMPO-oxidized cellulose nanofibrils, a material with known shear thinning behavior. Hydrogels presented tragacanth gum (TG) concentration-dependent rheological properties suitable for extrusion 3D printing. TG enhanced the swelling capacity up to 645% and the degradation rate up to 1.3%/day for hydrogels containing 75% of TG. Young's moduli of the hydrogels varied from 5.0 to 11.6 kPa and were comparable to soft tissues like skin and muscle. In vitro cell viability assays revealed that the scaffolds were non-toxic and promoted proliferation of hepatocellular carcinoma HepG2 cells. Therefore, the plant-based hydrogels designed in this work have a significant potential for tissue engineering.

AB - Producing hydrogels capable of mimicking the biomechanics of soft tissue remains a challenge. We explore the potential of plant-based hydrogels as polysaccharide tragacanth gum and antioxidant lignin nanoparticles in bioactive multicomponent hydrogels for tissue engineering. These natural components are combined with TEMPO-oxidized cellulose nanofibrils, a material with known shear thinning behavior. Hydrogels presented tragacanth gum (TG) concentration-dependent rheological properties suitable for extrusion 3D printing. TG enhanced the swelling capacity up to 645% and the degradation rate up to 1.3%/day for hydrogels containing 75% of TG. Young's moduli of the hydrogels varied from 5.0 to 11.6 kPa and were comparable to soft tissues like skin and muscle. In vitro cell viability assays revealed that the scaffolds were non-toxic and promoted proliferation of hepatocellular carcinoma HepG2 cells. Therefore, the plant-based hydrogels designed in this work have a significant potential for tissue engineering.

KW - Tragacanth gum

KW - Hydrogels

KW - Cellulose nanofibrils

KW - Lignin nanoparticles (LNP)

KW - Bioprinting

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JO - International Journal of Biological Macromolecules

JF - International Journal of Biological Macromolecules

ER -

Biological activity of multicomponent bio-hydrogels loaded with tragacanth gum

Abstract

Keywords

UN SDGs

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Biological activity of multicomponent bio-hydrogels loaded with tragacanth gum

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UN SDGs

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