Bioethanol production from wheat straw by phosphoric acid plus hydrogen peroxide (PHP) pretreatment via simultaneous saccharification and fermentation (SSF) at high solid loadings

Research output: Contribution to journalArticle


  • Jingwen Qiu
  • Dong Tian
  • Fei Shen
  • Jinguang Hu
  • Yongmei Zeng
  • Gang Yang
  • Yanzong Zhang
  • Shihuai Deng
  • Jing Zhang

Research units

  • Sichuan Agricultural University
  • University of British Columbia


Phosphoric acid plus hydrogen peroxide (PHP) pretreatment was employed on wheat straw for ethanol conversion by simultaneous saccharification and fermentation (SSF) at high loadings. Results showed solid loading of PHP-pretreated wheat straw can be greatly promoted to 20%. Although more enzyme input improved ethanol conversion significantly, it still can be potentially reduced to 10–20 mg protein/g cellulose. Increasing yeast input also promoted ethanol conversion, however, the responses were not significant. Response surface method was employed to optimize SSF conditions with the strategy of maximizing ethanol conversion and concentration and minimizing enzyme and yeast input. Results indicated that ethanol conversion of 88.2% and concentration of 69.9 g/L were obtained after 120 h SSF at solid loading of 15.3%, and CTec2 enzyme and yeast were in lower input of 13.2 mg protein/g cellulose and 1.0 g/L, respectively. Consequently, 15.5 g ethanol was harvested from 100 g wheat straw in the optimal conditions.


Original languageEnglish
Pages (from-to)355-362
Number of pages8
JournalBioresource Technology
Publication statusPublished - 1 Nov 2018
MoE publication typeA1 Journal article-refereed

    Research areas

  • Bioethanol, High solid loadings, Phosphoric acid plus hydrogen peroxide, Response surface method, Simultaneous saccharification and fermentation

ID: 27528128