Bioconversion of D-xylose to D-xylonate with Kluyveromyces lactis

Yvonne Nygard*, Mervi H. Toivari, Merja Penttilae, Laura Ruohonen, Marilyn G. Wiebe

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

Abstract

D-Xylonate was produced from D-xylose using Kluyveromyces lactis strains which expressed the gene for NADP(+)-dependent D-xylose dehydrogenase from Trichoderma reesei (xyd1). Up to 19 +/- 2 g D-xylonate l(-1) was produced when K. lactis expressing xyd1 was grown on 10.5 g D-galactose l (1) and 40 g D-xylose l (1). Intracellular accumulation of D-xylonate (up to similar to 70 mg [g biomass](-1)) was observed.

D-Xylose was metabolised to D-xylonate, xylitol and biomass. Oxygen could be reduced to 6 mmol O(2) l(-1) h(-1) without loss in titre or production rate, but metabolism of D-xylose and xylitol were more efficient when 12 mmol O(2) l(-1) h(-1) were provided.

D-Xylose uptake was not affected by deletion of either the D-xylose reductase (XYL1) or a putative xylitol dehydrogenase encoding gene (XYL2) in xyd1 expressing strains. K. lactis xyd1 Delta XYL1 did not produce extracellular xylitol and produced more D-xylonate than the xyd1 strain containing the endogenous XYL1. K. lactis xyd1 Delta XYL2 produced high concentrations of xylitol and significantly less D-xylonate than the xyd1 strain with the endogenous XYL2. (C) 2011 Elsevier Inc. All rights reserved.

Original languageEnglish
Pages (from-to)383-391
Number of pages9
JournalMETABOLIC ENGINEERING
Volume13
Issue number4
DOIs
Publication statusPublished - Jul 2011
MoE publication typeA1 Journal article-refereed

Keywords

  • D-xylonic acid
  • D-xylose
  • K. lactis
  • Aeration
  • D-xylose reductase
  • Xylitol dehydrogenase
  • RECOMBINANT SACCHAROMYCES-CEREVISIAE
  • PSEUDOMONAS-FRAGI
  • GLUCONOBACTER-OXYDANS
  • HEXOSE TRANSPORTERS
  • CARBOXYLIC-ACIDS
  • YEAST
  • FERMENTATION
  • XYLITOL
  • DEHYDROGENASE
  • METABOLISM

Cite this

Nygard, Y., Toivari, M. H., Penttilae, M., Ruohonen, L., & Wiebe, M. G. (2011). Bioconversion of D-xylose to D-xylonate with Kluyveromyces lactis. METABOLIC ENGINEERING, 13(4), 383-391. https://doi.org/10.1016/j.ymben.2011.04.001