Bioaugmentation enhances dark fermentative hydrogen production in cultures exposed to short-term temperature fluctuations

Onyinye Okonkwo*, Renaud Escudie, Nicolas Bernet, Rahul Mangayil, Aino Maija Lakaniemi, Eric Trably

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

17 Citations (Scopus)

Abstract

Hydrogen-producing mixed cultures were subjected to a 48-h downward or upward temperature fluctuation from 55 to 35 or 75 °C. Hydrogen production was monitored during the fluctuations and for three consecutive batch cultivations at 55 °C to evaluate the impact of temperature fluctuations and bioaugmentation with synthetic mixed culture of known H2 producers either during or after the fluctuation. Without augmentation, H2 production was significantly reduced during the downward temperature fluctuation and no H2 was produced during the upward fluctuation. H2 production improved significantly during temperature fluctuation when bioaugmentation was applied to cultures exposed to downward or upward temperatures. However, when bioaugmentation was applied after the fluctuation, i.e., when the cultures were returned to 55 °C, the H2 yields obtained were between 1.6 and 5% higher than when bioaugmentation was applied during the fluctuation. Thus, the results indicate the usefulness of bioaugmentation in process recovery, especially if bioaugmentation time is optimised.

Original languageEnglish
Pages (from-to)439-449
Number of pages11
JournalApplied Microbiology and Biotechnology
Volume104
Issue number1
DOIs
Publication statusPublished - 1 Jan 2020
MoE publication typeA1 Journal article-refereed

Keywords

  • Bioaugmentation time
  • Biohydrogen
  • Process recovery
  • Resilience
  • Synthetic mixed culture

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