Bioactive Properties of the Aqueous Extracts of Endophytic Fungi Associated with Scots Pine (Pinus sylvestris) Roots

Jenni Tienaho, Maarit Karonen, Riina Muilu-Mäkelä, Janne Kaseva, Nuria de Pedro, Francisca Vicente, Olga Genilloud, Ulla Aapola, Hannu Uusitalo, Katriina Vuolteenaho, Robert Franzén, Kristiina Wähälä, Matti Karp, Ville Santala, Tytti Sarjala

Research output: Contribution to journalArticleScientificpeer-review

1 Citation (Scopus)

Abstract

Despite the continuing interest in various plant and natural products, only a small portion of the biologically active compounds from nature has been discovered and exploited. In this study, antioxidant and antibacterial properties of aqueous fractions of three endophytic fungi isolated from the roots of 8-year-old Scots pines (Pinus sylvestris) growing on a drained peatland were investigated. The endophytic fungi species were Acephala applanata, Phialocephala fortinii, and Humicolopsis cephalosporioides/Coniochaeta mutabilis. The bioactivities were examined using hydrogen peroxide scavenging and oxygen radical absorbance capacity tests as well as sensitive Escherichia coli-based biosensors, which produce a luminescent signal in the presence of substances with oxidative or genotoxic properties. In addition, cell models for Parkinson's disease, age-related macular degeneration, and osteoarthritis were used to evaluate the potential for pharmaceutical applications. The aqueous extracts of fungi and 19 out of 42 fractions were found to be active in one or more of the tests used. However, no activity was found in the age-related macular degeneration and osteoarthritis cell model tests. Additionally, bioactivity data was connected with metabolites putatively annotated, and out of 330 metabolites, 177 were interesting in view of the bioactivities investigated. A majority of these were peptides and all three fungal species shared a highly similar metabolome. We propose that Scots pine endophytic fungi are a rich source of interesting metabolites, and synergistic effects may cause the bioactivities, as they were found to vary after the fractionation process.

Original languageEnglish
Pages (from-to)1009-1024
Number of pages16
JournalPlanta Medica
Volume86
Issue number13-14
DOIs
Publication statusPublished - 1 Sep 2020
MoE publication typeA1 Journal article-refereed

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