Bioactive Cellulose Nanofibrils for Specific Human IgG Binding

Yanxia Zhang*, Ruben G. Carbonell, Orlando J. Rojas

*Corresponding author for this work

    Research output: Contribution to journalArticleScientificpeer-review

    47 Citations (Scopus)

    Abstract

    Bioactive films were produced by conjugation of a short peptide onto modified cellulose nanofibrils (CNF). Specifically, a hydrophilic copolymer, poly(2-aminoethyl methacrylate hydrochloride-co-2-hydroxyethyl-methacrylate) (poly(AMA-co-HEMA)), was grafted via surface initiated polymerization from an initiator coupled to CNF. The poly(AMA-co-HEMA) was used as a spacer and support layer for immobilization of the peptide, acetylated-HWRGWVA, which has specific affinity with human immunoglobulin G (hIgG). Two methods for peptide grafting were compared: modification of CNF in aqueous suspension followed by assembly into a bioactive film and peptide grafting on a preformed CNF film. The CNF-based networks were examined on solid supports via atomic force microscopy (AFM) and extreme resolution imaging with ultralow electron landing energies (scanning low energy electron microscopy). The specific binding capability of hIgG and nonspecific protein resistance of the resultant peptide-modified CNF were evaluated by using quartz crystal microgravimetry (QCM). The effects of initiator concentration and thickness of poly(AMA-co-HEMA) layer on hIgG adsorption were investigated in the developed systems, which exhibited high signal-to-noise response.

    Original languageEnglish
    Pages (from-to)4161-4168
    Number of pages8
    JournalBiomacromolecules
    Volume14
    Issue number12
    DOIs
    Publication statusPublished - Dec 2013
    MoE publication typeA1 Journal article-refereed

    Keywords

    • TRANSFER RADICAL POLYMERIZATION
    • HUMAN-IMMUNOGLOBULIN G
    • MICROFIBRILLATED CELLULOSE
    • NATIVE CELLULOSE
    • PEPTIDE LIGANDS
    • CELL-CULTURE
    • SURFACE
    • PURIFICATION
    • BRUSHES
    • DENSITY

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