TY - JOUR
T1 - Bio-Piezoelectric Ceramic Composites for Electroactive Implants—Biological Performance
AU - Fernandes, Beatriz Ferreira
AU - Silva, Neusa
AU - Marques, Joana Faria
AU - Da Cruz, Mariana Brito
AU - Tiainen, Laura
AU - Gasik, Michael
AU - Carvalho, Óscar
AU - Silva, Filipe Samuel
AU - Caramês, João
AU - Mata, António
N1 - Funding Information:
This research was funded by FCT project POCI-01-0145-FEDER-030498—Portugal, by FEDER funds through the COMPETE 2020—Programa Operacional Competitividade e Internacionalização (POCI). Partially funded by the Faculty of Dental Medicine of the University of Lisbon Scholarship for Open Access Publications.
Publisher Copyright:
© 2023 by the authors.
PY - 2023/8/1
Y1 - 2023/8/1
N2 - Barium titanate (BaTiO3) piezoelectric ceramic may be a potential alternative for promoting osseointegration due to its piezoelectric properties similar to bone electric potentials generated in loading function. In this sense, the aim of this in vitro study was to evaluate the cellular response of human osteoblasts and gingival fibroblasts as well as the impact on S. oralis when in contact with BaTiO3 functionalized zirconia implant surfaces with piezoelectric properties. Zirconia discs with BaTiO3 were produced and contact poling (piezo activation) was performed. Osteoblasts (hFOB 1.19), fibroblasts (HGF hTERT) and S. oralis were culture on discs. Cell viability and morphology, cell differentiation markers, bacterial adhesion and growth were evaluated. The present study suggests that zirconia composite surfaces with the addition of piezoelectric BaTiO3 are not cytotoxic to peri-implant cells. Also, they seem to promote a faster initial osteoblast differentiation. Moreover, these surfaces may inhibit the growth of S. oralis by acting as a bacteriostatic agent over time. Although the piezoelectric properties do not affect the cellular inflammatory profile, they appear to enable the initial adhesion of bacteria, however this is not significant over the entire testing period. Furthermore, the addition of non-poled BaTiO3 to zirconia may have a potential reduction effect on IL-6 mediated-inflammatory activity in fibroblasts.
AB - Barium titanate (BaTiO3) piezoelectric ceramic may be a potential alternative for promoting osseointegration due to its piezoelectric properties similar to bone electric potentials generated in loading function. In this sense, the aim of this in vitro study was to evaluate the cellular response of human osteoblasts and gingival fibroblasts as well as the impact on S. oralis when in contact with BaTiO3 functionalized zirconia implant surfaces with piezoelectric properties. Zirconia discs with BaTiO3 were produced and contact poling (piezo activation) was performed. Osteoblasts (hFOB 1.19), fibroblasts (HGF hTERT) and S. oralis were culture on discs. Cell viability and morphology, cell differentiation markers, bacterial adhesion and growth were evaluated. The present study suggests that zirconia composite surfaces with the addition of piezoelectric BaTiO3 are not cytotoxic to peri-implant cells. Also, they seem to promote a faster initial osteoblast differentiation. Moreover, these surfaces may inhibit the growth of S. oralis by acting as a bacteriostatic agent over time. Although the piezoelectric properties do not affect the cellular inflammatory profile, they appear to enable the initial adhesion of bacteria, however this is not significant over the entire testing period. Furthermore, the addition of non-poled BaTiO3 to zirconia may have a potential reduction effect on IL-6 mediated-inflammatory activity in fibroblasts.
KW - barium titanate
KW - dental implants
KW - fibroblasts
KW - osteoblasts
KW - piezoelectric properties
KW - zirconia
UR - http://www.scopus.com/inward/record.url?scp=85169042942&partnerID=8YFLogxK
U2 - 10.3390/biomimetics8040338
DO - 10.3390/biomimetics8040338
M3 - Article
AN - SCOPUS:85169042942
SN - 2313-7673
VL - 8
JO - Biomimetics
JF - Biomimetics
IS - 4
M1 - 338
ER -