Bacterial community structure and enzyme activities in a membrane bioreactor (MBR) using pure oxygen as an aeration source

Research output: Contribution to journalArticleScientificpeer-review


  • Kadiya Calderón
  • Alejandro González-Martínez
  • Camino Montero-Puente
  • Patricia Reboleiro-Rivas
  • José M. Poyatos
  • Belén Juárez-Jiménez
  • María Victoria Martínez-Toledo
  • Belén Rodelas

Research units

  • University of Granada


A pilot-scale membrane bioreactor was used to treat urban wastewater using pure oxygen instead of air as a source of aeration, to study its influence on bacterial diversity and levels of enzyme activities (acid and alkaline phosphatases, glucosidase, protease, and esterase) in the sludge. The experimental work was developed in two stages influenced by seasonal temperature. Operational parameters (temperature, pH, BOD 5, COD, total and volatile suspended solids) were daily monitored, and enzyme activities measured twice a week. Redundancy analysis (RDA) was used to reveal relationships between the level of enzyme activities and the variation of operational parameters, demonstrating a significant effect of temperature and volatile suspended solids. Bacterial diversity was analyzed by temperature-gradient gel electrophoresis of PCR-amplified partial 16S rRNA genes. Significant differences in community structure were observed between both stages. Sequence analysis revealed that the prevalent Bacteria populations were evolutively close to Alphaproteobacteria (44%), Betaproteobacteria (25%) and Firmicutes (17%).


Original languageEnglish
Pages (from-to)87-94
Number of pages8
JournalBioresource Technology
Issue number1
Publication statusPublished - Jan 2012
MoE publication typeA1 Journal article-refereed

    Research areas

  • Enzyme activities, MBR, Membrane bioreactor, Pure oxygen, TGGE

ID: 8886258