Bacillus subtilis and B-mojavensis strains connected to food poisoning produce the heat stable toxin amylosin

Research output: Contribution to journalArticleScientificpeer-review

Researchers

Research units

  • Thermo Fisher Scientific Oy
  • University of Helsinki
  • Russian Academy of Sciences
  • Finnish Food Safety Authority (EVIRA)

Abstract

Aim: To screen and characterize toxic, heat-stable substances produced by food borne strains from Bacillus subtilis group.

Methods and Results: Using the boar sperm motility inhibition assay, six isolates from two outbreaks, out of the 94 isolates from 26 foods, were found to produce ethanol-soluble heat-stable substances that were toxic to sperm cells by depleting the mitochondrial membrane potentials. The toxic isolates were identified as Bacillus subtilis and B. mojavensis. Colon carcinoma cells (Caco-2) were used to model the contact with the human digestive tract. The extract of B. subtilis F 2564/96 depolarized the mitochondria in intact Caco-2 cells similarly as in sperm cells. The substance responsible for these effects was purified using HPLC and identified by electron spray ionization ion trap mass spectrometry analysis as amylosin. The temperature requirement for amylosin production was 21-37 degrees C for B. subtilis and 11-21 degrees C for B. mojavensis. Both species produced amylosin in air as well as in 7-8% CO(2) with 8-9% O(2).

Conclusions: Food borne illness related strains of B. subtilis and B. mojavensis, produced the heat-stable toxin amylosin.

Significance and Impact of the Study: This is the first report that suggests a role for the heat-stable, ion-channel forming toxin amylosin, as a virulence factor in food borne Bacillus.

Details

Original languageEnglish
Pages (from-to)1976-1985
Number of pages10
JournalJournal of Applied Microbiology
Volume106
Issue number6
Publication statusPublished - Jun 2009
MoE publication typeA1 Journal article-refereed

    Research areas

  • amylosin, Bacillus subtilis, Bacillus mojavensis, food borne, CEREUS GROUP, SPP., LICHENIFORMIS, CONTAMINANTS, ENVIRONMENT, SURFACTIN, TOXICITY, BIOASSAY, PUMILUS, MILK

ID: 7090469