Automation of the Resazurin Reduction Test using Fluorometry of Microtitration Trays

T. Ali‐Vehmas*, Louhi M., M. Sandholm

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

23 Citations (Scopus)

Abstract

Microtitration plate technique and ‐fluorometry was applied to automate the resazurin reduction test for monitoring bacterial numbers in broth cultures and milk. The effect of resazurin and resorufin concentration, bacterial species, growth medium, pH, and redox potential on the fluorescence response was studied. The timing of the appearance of maximum fluorescence was directly related to the logarithm of the number of colony‐forming units (log CFU). Fresh milk and heat‐treated milk contain interfering redox systems. The technique based on microtitration plate fluorometry, when fully automated, seems to provide a high‐capacity system for analyzing bacterial numbers in foodstuffs and other media.

Original languageEnglish
Pages (from-to)358-372
Number of pages15
JournalJournal of Veterinary Medicine. Series B
Volume38
Issue number1-10
DOIs
Publication statusPublished - 1991
MoE publication typeA1 Journal article-refereed

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