Automated Particle Collection for Protein Crystal Harvesting

Burak Zeydan; Andrew J. Petruska; Luca Somm; Roel Pieters; Yang Fang; David F. Sargent; Bradley J. Nelson

doi:10.1109/LRA.2017.2669364

Automated Particle Collection for Protein Crystal Harvesting

Burak Zeydan, Andrew J. Petruska, Luca Somm, Roel Pieters, Yang Fang, David F. Sargent, Bradley J. Nelson

Not published at Aalto University

Research output: Contribution to journal › Article › Scientific › peer-review

9 Citations (Scopus)

Abstract

A robust automated system to collect protein crystals for X-ray crystallography is presented. This system uses an ultraviolet imaging system based on commercial off-the-shelf components, a magnetically manipulated tool, and a resilient behavior-based controller. The system is validated by collecting over 350 polystyrene beads, used as crystal emulators, and transporting them 2 mm to a predefined goal in a 14-h period without human intervention. The average time to identify, collect, transport, and deliver a crystal emulator is 2.4 min, similar to an expert operator. This is the first demonstration of a completely automated robust system for protein crystal harvesting.

Original language	English
Article number	7856934
Pages (from-to)	1391-1396
Number of pages	6
Journal	IEEE Robotics and Automation Letters
Volume	2
Issue number	3
DOIs	https://doi.org/10.1109/LRA.2017.2669364
Publication status	Published - 1 Jul 2017
MoE publication type	A1 Journal article-refereed

Keywords

Automation at micro-nano scales
automation in life sciences: biotechnology
behaviour-based systems

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10.1109/LRA.2017.2669364

Cite this

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title = "Automated Particle Collection for Protein Crystal Harvesting",

abstract = "A robust automated system to collect protein crystals for X-ray crystallography is presented. This system uses an ultraviolet imaging system based on commercial off-the-shelf components, a magnetically manipulated tool, and a resilient behavior-based controller. The system is validated by collecting over 350 polystyrene beads, used as crystal emulators, and transporting them 2 mm to a predefined goal in a 14-h period without human intervention. The average time to identify, collect, transport, and deliver a crystal emulator is 2.4 min, similar to an expert operator. This is the first demonstration of a completely automated robust system for protein crystal harvesting.",

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doi = "10.1109/LRA.2017.2669364",

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AU - Zeydan, Burak

AU - Petruska, Andrew J.

AU - Somm, Luca

AU - Pieters, Roel

AU - Fang, Yang

AU - Sargent, David F.

AU - Nelson, Bradley J.

PY - 2017/7/1

Y1 - 2017/7/1

N2 - A robust automated system to collect protein crystals for X-ray crystallography is presented. This system uses an ultraviolet imaging system based on commercial off-the-shelf components, a magnetically manipulated tool, and a resilient behavior-based controller. The system is validated by collecting over 350 polystyrene beads, used as crystal emulators, and transporting them 2 mm to a predefined goal in a 14-h period without human intervention. The average time to identify, collect, transport, and deliver a crystal emulator is 2.4 min, similar to an expert operator. This is the first demonstration of a completely automated robust system for protein crystal harvesting.

AB - A robust automated system to collect protein crystals for X-ray crystallography is presented. This system uses an ultraviolet imaging system based on commercial off-the-shelf components, a magnetically manipulated tool, and a resilient behavior-based controller. The system is validated by collecting over 350 polystyrene beads, used as crystal emulators, and transporting them 2 mm to a predefined goal in a 14-h period without human intervention. The average time to identify, collect, transport, and deliver a crystal emulator is 2.4 min, similar to an expert operator. This is the first demonstration of a completely automated robust system for protein crystal harvesting.

KW - Automation at micro-nano scales

KW - automation in life sciences: biotechnology

KW - behaviour-based systems

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JO - IEEE Robotics and Automation Letters

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IS - 3

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ER -

Automated Particle Collection for Protein Crystal Harvesting

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Keywords

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