Assessing indoor air toxicity with condensate collected from air using the mitochondrial activity of human BJ fibroblasts and THP-1 monocytes

Heidi Salonen; Tuula Heinonen; Marika Mannerström; Mark Jackson; Maria Andersson; Raimo Mikkola; Jarek Kurnitski; Shahana Khurshid; Atila Novoselac; Richard Corsi

Assessing indoor air toxicity with condensate collected from air using the mitochondrial activity of human BJ fibroblasts and THP-1 monocytes

Heidi Salonen, Tuula Heinonen, Marika Mannerström, Mark Jackson, Maria Andersson, Raimo Mikkola, Jarek Kurnitski, Shahana Khurshid, Atila Novoselac, Richard Corsi

Department of Civil Engineering

Research output: Contribution to conference › Paper › Scientific › peer-review

Abstract

The hypothesis for our study was that air and airborne moisture are carriers of possible toxic compounds and these compounds can be collected as condensate using dry ice. Using human cells, we aimed to evaluate the indoor air toxic risk from the condensed water collected from several office and laboratory rooms in two different buildings, both located at the University of Texas at Austin. Condensate samples were collected by using stainless steel collectors (E-collectors), and the effects of samples on the viability of human BJ fibroblast and activated THP-1-monocytes in vitro were investigated by using WST-1 cytotoxicity assays. Toxicological assessment utilizing human BJ fibroblasts and activated THP-1-monocytes of condensate from air reflects the acute toxic effects of the indoor air. The first results suggest that toxicological assessment of condensate collected from air using dry ice in stainless steel condensate collectors and human BJ fibroblasts and activated THP-1-monocytes as test organisms could be useful in detecting indoor air anomalies.

Original language	English
Publication status	Published - 22 Jul 2018
MoE publication type	Not Eligible
Event	Conference of the International Society of Indoor Air Quality and Climate - Philadelphia , United States Duration: 22 Jul 2018 → 27 Jul 2018 Conference number: 15 http://www.indoorair2018.org/

Conference

Conference	Conference of the International Society of Indoor Air Quality and Climate
Abbreviated title	ISIAQ
Country/Territory	United States
City	Philadelphia
Period	22/07/2018 → 27/07/2018
Internet address	http://www.indoorair2018.org/

SIBI: Sisätiloissa käytettyjen siivouskemikaalien ja biosidien vaikutukset mitattuun ja koettuun sisäilman laatuun koulu- ja päiväkotirakennuksissa
Salonen, H. (Principal investigator), Castagnoli, E. (Project Member), Järvi, K. (Project Member), Mikkola, R. (Project Member), Vornanen-Winqvist, C. (Project Member) & Alapieti, T. (Project Member)
01/09/2017 → 31/12/2019
Project: Domestic funds and foundations
Toxin transport mechanisms from moisture damaged structures to indoor air
Castagnoli, E. (Project Member), Kurnitski, J. (Principal investigator), Mikkola, R. (Project Member), Piironen, J. (Project Member), Taebnia, M. (Project Member), Alapieti, T. (Project Member), Järvi, K. (Project Member), Salonen, H. (Project Member), Vornanen-Winqvist, C. (Project Member) & Ahmed, K. (Project Member)
01/09/2015 → 31/08/2019
Project: Academy of Finland: Other research funding

Cite this

Salonen, H., Heinonen, T., Mannerström, M., Jackson, M., Andersson, M., Mikkola, R., Kurnitski, J., Khurshid, S., Novoselac, A., & Corsi, R. (2018). Assessing indoor air toxicity with condensate collected from air using the mitochondrial activity of human BJ fibroblasts and THP-1 monocytes. Paper presented at Conference of the International Society of Indoor Air Quality and Climate , Philadelphia , Pennsylvania, United States.

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title = "Assessing indoor air toxicity with condensate collected from air using the mitochondrial activity of human BJ fibroblasts and THP-1 monocytes",

abstract = "The hypothesis for our study was that air and airborne moisture are carriers of possible toxic compounds and these compounds can be collected as condensate using dry ice. Using human cells, we aimed to evaluate the indoor air toxic risk from the condensed water collected from several office and laboratory rooms in two different buildings, both located at the University of Texas at Austin. Condensate samples were collected by using stainless steel collectors (E-collectors), and the effects of samples on the viability of human BJ fibroblast and activated THP-1-monocytes in vitro were investigated by using WST-1 cytotoxicity assays. Toxicological assessment utilizing human BJ fibroblasts and activated THP-1-monocytes of condensate from air reflects the acute toxic effects of the indoor air. The first results suggest that toxicological assessment of condensate collected from air using dry ice in stainless steel condensate collectors and human BJ fibroblasts and activated THP-1-monocytes as test organisms could be useful in detecting indoor air anomalies.",

author = "Heidi Salonen and Tuula Heinonen and Marika Mannerstr{\"o}m and Mark Jackson and Maria Andersson and Raimo Mikkola and Jarek Kurnitski and Shahana Khurshid and Atila Novoselac and Richard Corsi",

note = "Paper ID: 330; Conference of the International Society of Indoor Air Quality and Climate , ISIAQ ; Conference date: 22-07-2018 Through 27-07-2018",

year = "2018",

month = jul,

day = "22",

language = "English",

url = "http://www.indoorair2018.org/",

}

Salonen, H, Heinonen, T, Mannerström, M, Jackson, M, Andersson, M , Mikkola, R , Kurnitski, J, Khurshid, S, Novoselac, A & Corsi, R 2018, 'Assessing indoor air toxicity with condensate collected from air using the mitochondrial activity of human BJ fibroblasts and THP-1 monocytes', Paper presented at Conference of the International Society of Indoor Air Quality and Climate , Philadelphia , United States, 22/07/2018 - 27/07/2018.

Assessing indoor air toxicity with condensate collected from air using the mitochondrial activity of human BJ fibroblasts and THP-1 monocytes. / Salonen, Heidi; Heinonen, Tuula; Mannerström, Marika et al.
2018. Paper presented at Conference of the International Society of Indoor Air Quality and Climate , Philadelphia , Pennsylvania, United States.

Research output: Contribution to conference › Paper › Scientific › peer-review

TY - CONF

T1 - Assessing indoor air toxicity with condensate collected from air using the mitochondrial activity of human BJ fibroblasts and THP-1 monocytes

AU - Salonen, Heidi

AU - Heinonen, Tuula

AU - Mannerström, Marika

AU - Jackson, Mark

AU - Andersson, Maria

AU - Mikkola, Raimo

AU - Kurnitski, Jarek

AU - Khurshid, Shahana

AU - Novoselac, Atila

AU - Corsi, Richard

N1 - Conference code: 15

PY - 2018/7/22

Y1 - 2018/7/22

N2 - The hypothesis for our study was that air and airborne moisture are carriers of possible toxic compounds and these compounds can be collected as condensate using dry ice. Using human cells, we aimed to evaluate the indoor air toxic risk from the condensed water collected from several office and laboratory rooms in two different buildings, both located at the University of Texas at Austin. Condensate samples were collected by using stainless steel collectors (E-collectors), and the effects of samples on the viability of human BJ fibroblast and activated THP-1-monocytes in vitro were investigated by using WST-1 cytotoxicity assays. Toxicological assessment utilizing human BJ fibroblasts and activated THP-1-monocytes of condensate from air reflects the acute toxic effects of the indoor air. The first results suggest that toxicological assessment of condensate collected from air using dry ice in stainless steel condensate collectors and human BJ fibroblasts and activated THP-1-monocytes as test organisms could be useful in detecting indoor air anomalies.

AB - The hypothesis for our study was that air and airborne moisture are carriers of possible toxic compounds and these compounds can be collected as condensate using dry ice. Using human cells, we aimed to evaluate the indoor air toxic risk from the condensed water collected from several office and laboratory rooms in two different buildings, both located at the University of Texas at Austin. Condensate samples were collected by using stainless steel collectors (E-collectors), and the effects of samples on the viability of human BJ fibroblast and activated THP-1-monocytes in vitro were investigated by using WST-1 cytotoxicity assays. Toxicological assessment utilizing human BJ fibroblasts and activated THP-1-monocytes of condensate from air reflects the acute toxic effects of the indoor air. The first results suggest that toxicological assessment of condensate collected from air using dry ice in stainless steel condensate collectors and human BJ fibroblasts and activated THP-1-monocytes as test organisms could be useful in detecting indoor air anomalies.

M3 - Paper

T2 - Conference of the International Society of Indoor Air Quality and Climate

Y2 - 22 July 2018 through 27 July 2018

ER -

Assessing indoor air toxicity with condensate collected from air using the mitochondrial activity of human BJ fibroblasts and THP-1 monocytes

Abstract

Conference

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Projects

SIBI: Sisätiloissa käytettyjen siivouskemikaalien ja biosidien vaikutukset mitattuun ja koettuun sisäilman laatuun koulu- ja päiväkotirakennuksissa

Toxin transport mechanisms from moisture damaged structures to indoor air

Cite this