Artificial electron acceptors decouple archaeal methane oxidation from sulfate reduction

Silvan Scheller*, Hang Yu, Grayson L. Chadwick, Shawn E. McGlynn, Victoria J. Orphan

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

173 Citations (Scopus)

Abstract

The oxidation of methane with sulfate is an important microbial metabolism in the global carbon cycle. In marine methane seeps, this process is mediated by consortia of anaerobic methanotrophic archaea (ANME) that live in syntrophy with sulfate-reducing bacteria (SRB). The underlying interdependencies within this uncultured symbiotic partnership are poorly understood. We used a combination of rate measurements and single-cell stable isotope probing to demonstrate that ANME in deep-sea sediments can be catabolically and anabolically decoupled from their syntrophic SRB partners using soluble artificial oxidants. The ANME still sustain high rates of methane oxidation in the absence of sulfate as the terminal oxidant, lending support to the hypothesis that interspecies extracellular electron transfer is the syntrophic mechanism for the anaerobic oxidation of methane.

Original languageEnglish
Pages (from-to)703-707
Number of pages5
JournalScience
Volume351
Issue number6274
DOIs
Publication statusPublished - 12 Feb 2016
MoE publication typeA1 Journal article-refereed

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