Array-based disease diagnostics using lipid/polydiacetylene vesicles encapsulated in a sol-gel matrix

Research output: Contribution to journalArticle

Researchers

  • Sofiya Kolusheva
  • R. Yossef
  • A. Kugel
  • M. Katz
  • R. Volinsky
  • M. Welt
  • U. Hadad
  • V. Drory
  • M. Kliger
  • E. Rubin
  • A. Porgador
  • Raz Jelinek

Research units

  • Ben-Gurion University of the Negev
  • Tel Aviv University
  • Medasense Biometrics Ltd.

Abstract

We demonstrate a novel array-based diagnostic platform comprising lipid/polydiacetylene (PDA) vesicles embedded within a transparent silica-gel matrix. The diagnostic scheme is based upon the unique chromatic properties of PDA, which undergoes blue-red transformations induced by interactions with amphiphilic or membrane-active analytes. We show that constructing a gel matrix array hosting PDA vesicles with different lipid compositions and applying to blood plasma obtained from healthy individuals and from patients suffering from disease, respectively, allow distinguishing among the disease conditions through application of a simple machine-learning algorithm, using the colorimetric response of the lipid/PDA/gel matrix as the input. Importantly, the new colorimetric diagnostic approach does not require a priori knowledge on the exact metabolite compositions of the blood plasma, since the concept relies only on identifying statistically significant changes in overall disease-induced chromatic response. The chromatic lipid/PDA/gel array-based " fingerprinting" concept is generic, easy to apply, and could be implemented for varied diagnostic and screening applications.

Details

Original languageEnglish
Pages (from-to)5925-5931
Number of pages7
JournalAnalytical Chemistry
Volume84
Issue number14
Publication statusPublished - 17 Jul 2012
MoE publication typeA1 Journal article-refereed

ID: 13537078