Are solar UV-B- and UV-A-dependent gene expression and metabolite accumulation in Arabidopsis mediated by the stress response regulator RADICAL-INDUCED CELL DEATH1?

Luis O. Morales*, Mikael Brosché, Julia P. Vainonen, Nina Sipari, Anders V. Lindfors, Åke Strid, Pedro J. Aphalo

*Corresponding author for this work

    Research output: Contribution to journalArticleScientificpeer-review

    8 Citations (Scopus)

    Abstract

    Wavelengths in the ultraviolet (UV) region of the solar spectrum, UV-B (280-315nm) and UV-A (315-400nm), are key environmental signals modifying several aspects of plant physiology. Despite significant advances in the understanding of plant responses to UV-B and the identification of signalling components involved, there is limited information on the molecular mechanisms that control UV-B signalling in plants under natural sunlight. Here, we aimed to corroborate the previous suggested role for RADICAL-INDUCED CELL DEATH1 (RCD1) in UV-B signalling under full spectrum sunlight. Wild-type Arabidopsis thaliana and the rcd1-1 mutant were used in an experimental design outdoors where UV-B and UV-A irradiances were manipulated using plastic films, and gene expression, PYRIDOXINE BIOSYNTHESIS1 (PDX1) accumulation and metabolite profiles were analysed in the leaves. At the level of transcription, RCD1 was not directly involved in the solar UV-B regulation of genes with functions in UV acclimation, hormone signalling and stress-related markers. Furthermore, RCD1 had no role on PDX1 accumulation but modulated the UV-B induction of flavonoid accumulation in leaves of Arabidopsis exposed to solar UV. We conclude that RCD1 does not play an active role in UV-B signalling but rather modulates UV-B responses under full spectrum sunlight.

    Original languageEnglish
    Pages (from-to)878-891
    Number of pages14
    JournalPLANT CELL AND ENVIRONMENT
    Volume38
    Issue number5
    DOIs
    Publication statusPublished - 1 May 2015
    MoE publication typeA1 Journal article-refereed

    Keywords

    • Flavonoids
    • RCD1
    • Signalling
    • Solar UV
    • Stress
    • UV acclimation

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