Aptamer-embedded DNA origami cage for detecting (glycated) hemoglobin with a surface plasmon resonance sensor

Surachet Duanghathaipornsuk; Boxuan Shen; Brent D. Cameron; Heini Ijäs; Veikko Linko; Mauri Kostiainen; Dong-Shik Kim

doi:10.1016/j.matlet.2020.128141

Aptamer-embedded DNA origami cage for detecting (glycated) hemoglobin with a surface plasmon resonance sensor

Surachet Duanghathaipornsuk, Boxuan Shen, Brent D. Cameron, Heini Ijäs, Veikko Linko, Mauri Kostiainen, Dong-Shik Kim^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Scientific › peer-review

4 Citations (Scopus)

Abstract

DNA origami-based cages functionalized with aptamer motifs were used to detect hemoglobin and glycated hemoglobin. The binding between the cages and hemoglobin was monitored using a surface plasmon resonance (SPR) sensor. One DNA strand in the nano-cage was replaced with an aptamer that demonstrated a high affinity to hemoglobin (Hb) or glycated hemoglobin (gHb). Three types of the DNA nano-cages designed to fit the size and shape of hemoglobin were evaluated: one without an aptamer, one with the Hb-affinity aptamer (HA) and one with the gHb-affinity aptamer (GHA). Both DNA nano-cages embedded with HA and GHA showed significantly more stable binding with Hb and gHb by 5 and 9 times, respectively, than the aptamers directly immobilized on the SPR surface. HA-embedded DNA and GHA-embedded DNA improved the sensor selectivities by 9 times and 37 times between Hb and gHb.

Original language	English
Article number	128141
Number of pages	4
Journal	Materials Letters
Volume	275
DOIs	https://doi.org/10.1016/j.matlet.2020.128141
Publication status	Published - 15 Sep 2020
MoE publication type	A1 Journal article-refereed

Keywords

Aptamer
Dissociation constant
DNA origami
Glycated hemoglobin
Surface plasmon resonance sensor

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10.1016/j.matlet.2020.128141

http://urn.fi/URN:NBN:fi:jyu-202006234420

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title = "Aptamer-embedded DNA origami cage for detecting (glycated) hemoglobin with a surface plasmon resonance sensor",

abstract = "DNA origami-based cages functionalized with aptamer motifs were used to detect hemoglobin and glycated hemoglobin. The binding between the cages and hemoglobin was monitored using a surface plasmon resonance (SPR) sensor. One DNA strand in the nano-cage was replaced with an aptamer that demonstrated a high affinity to hemoglobin (Hb) or glycated hemoglobin (gHb). Three types of the DNA nano-cages designed to fit the size and shape of hemoglobin were evaluated: one without an aptamer, one with the Hb-affinity aptamer (HA) and one with the gHb-affinity aptamer (GHA). Both DNA nano-cages embedded with HA and GHA showed significantly more stable binding with Hb and gHb by 5 and 9 times, respectively, than the aptamers directly immobilized on the SPR surface. HA-embedded DNA and GHA-embedded DNA improved the sensor selectivities by 9 times and 37 times between Hb and gHb.",

keywords = "Aptamer, Dissociation constant, DNA origami, Glycated hemoglobin, Surface plasmon resonance sensor",

author = "Surachet Duanghathaipornsuk and Boxuan Shen and Cameron, {Brent D.} and Heini Ij{\"a}s and Veikko Linko and Mauri Kostiainen and Dong-Shik Kim",

year = "2020",

month = sep,

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doi = "10.1016/j.matlet.2020.128141",

language = "English",

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AU - Duanghathaipornsuk, Surachet

AU - Shen, Boxuan

AU - Cameron, Brent D.

AU - Ijäs, Heini

AU - Linko, Veikko

AU - Kostiainen, Mauri

AU - Kim, Dong-Shik

PY - 2020/9/15

Y1 - 2020/9/15

N2 - DNA origami-based cages functionalized with aptamer motifs were used to detect hemoglobin and glycated hemoglobin. The binding between the cages and hemoglobin was monitored using a surface plasmon resonance (SPR) sensor. One DNA strand in the nano-cage was replaced with an aptamer that demonstrated a high affinity to hemoglobin (Hb) or glycated hemoglobin (gHb). Three types of the DNA nano-cages designed to fit the size and shape of hemoglobin were evaluated: one without an aptamer, one with the Hb-affinity aptamer (HA) and one with the gHb-affinity aptamer (GHA). Both DNA nano-cages embedded with HA and GHA showed significantly more stable binding with Hb and gHb by 5 and 9 times, respectively, than the aptamers directly immobilized on the SPR surface. HA-embedded DNA and GHA-embedded DNA improved the sensor selectivities by 9 times and 37 times between Hb and gHb.

AB - DNA origami-based cages functionalized with aptamer motifs were used to detect hemoglobin and glycated hemoglobin. The binding between the cages and hemoglobin was monitored using a surface plasmon resonance (SPR) sensor. One DNA strand in the nano-cage was replaced with an aptamer that demonstrated a high affinity to hemoglobin (Hb) or glycated hemoglobin (gHb). Three types of the DNA nano-cages designed to fit the size and shape of hemoglobin were evaluated: one without an aptamer, one with the Hb-affinity aptamer (HA) and one with the gHb-affinity aptamer (GHA). Both DNA nano-cages embedded with HA and GHA showed significantly more stable binding with Hb and gHb by 5 and 9 times, respectively, than the aptamers directly immobilized on the SPR surface. HA-embedded DNA and GHA-embedded DNA improved the sensor selectivities by 9 times and 37 times between Hb and gHb.

KW - Aptamer

KW - Dissociation constant

KW - DNA origami

KW - Glycated hemoglobin

KW - Surface plasmon resonance sensor

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DO - 10.1016/j.matlet.2020.128141

M3 - Article

VL - 275

JO - Materials Letters

JF - Materials Letters

SN - 0167-577X

M1 - 128141

ER -

Aptamer-embedded DNA origami cage for detecting (glycated) hemoglobin with a surface plasmon resonance sensor

Abstract

Keywords

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SYNVIRO: Synthetic Virology Toolbox for the Encapsulation of Therapeutic Polyelectrolytes (SYNVIRO)

Protein Cage Directed Self-Assembly of Nanomaterials

Rationally designed molecular devices through nucleic acid nanotechnology

OtaNano - Nanomicroscopy Center

Cite this

Aptamer-embedded DNA origami cage for detecting (glycated) hemoglobin with a surface plasmon resonance sensor

Abstract

Keywords

Access to Document

OpenUrl availability

Other files and links

Fingerprint

Projects

SYNVIRO: Synthetic Virology Toolbox for the Encapsulation of Therapeutic Polyelectrolytes (SYNVIRO)

Protein Cage Directed Self-Assembly of Nanomaterials

Rationally designed molecular devices through nucleic acid nanotechnology

Equipment

OtaNano - Nanomicroscopy Center

Cite this