Aptamer-embedded DNA origami cage for detecting (glycated) hemoglobin with a surface plasmon resonance sensor

Surachet Duanghathaipornsuk, Boxuan Shen, Brent D. Cameron, Heini Ijäs, Veikko Linko, Mauri Kostiainen, Dong-Shik Kim*

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

3 Citations (Scopus)

Abstract

DNA origami-based cages functionalized with aptamer motifs were used to detect hemoglobin and glycated hemoglobin. The binding between the cages and hemoglobin was monitored using a surface plasmon resonance (SPR) sensor. One DNA strand in the nano-cage was replaced with an aptamer that demonstrated a high affinity to hemoglobin (Hb) or glycated hemoglobin (gHb). Three types of the DNA nano-cages designed to fit the size and shape of hemoglobin were evaluated: one without an aptamer, one with the Hb-affinity aptamer (HA) and one with the gHb-affinity aptamer (GHA). Both DNA nano-cages embedded with HA and GHA showed significantly more stable binding with Hb and gHb by 5 and 9 times, respectively, than the aptamers directly immobilized on the SPR surface. HA-embedded DNA and GHA-embedded DNA improved the sensor selectivities by 9 times and 37 times between Hb and gHb.
Original languageEnglish
Article number128141
Number of pages4
JournalMaterials Letters
Volume275
DOIs
Publication statusPublished - 15 Sep 2020
MoE publication typeA1 Journal article-refereed

Keywords

  • Aptamer
  • Dissociation constant
  • DNA origami
  • Glycated hemoglobin
  • Surface plasmon resonance sensor

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