Antimycin A-producing nonphytopathogenic Streptomyces turgidiscabies from potato

Research output: Contribution to journalArticleScientificpeer-review

Researchers

Research units

  • University of Helsinki

Abstract

Aim: To detect if substances with mammalian cell toxicity are produced by Streptomyces turgidiscabies and Streptomyces scabiei isolated from potato scab lesions.

Methods and Results: In vitro cultures of phytopathogenic and nonphytopathogenic strains of S. scabiei and S. turgidiscabies, isolated from scab lesions of potato tubers originating from nine different cultivars from Finland and Sweden, were tested for toxicity using the rapid spermatozoan motility inhibition assay, previously shown useful in the detection of many different Streptomyces toxins and antimicrobial compounds. Purified toxins were used as reference. Three nonphytopathogenic strains of S. turgidiscabies were found to produce antimycin A when cultured on solid medium.

Conclusions: Boar sperm-motility-inhibiting substances are produced by strains of S. turgidiscabies and S. scabiei. The most powerful inhibitory substance, produced by three nonphytopathogenic S. turgidiscabies strains, was identified as antimycin A. The phytotoxic compounds thaxtomin A and concanamycin A did not inhibit sperm motility even at high doses.

Significance and Impact of the Study: The presence of antimycin A-producing Streptomyces strains, nonpathogenic to potato, was unexpected but important, considering the high mammalian toxicity of this cytochrome bc-blocking antibiotic.

Details

Original languageEnglish
Pages (from-to)1332-1340
Number of pages9
JournalJournal of Applied Microbiology
Volume104
Issue number5
Publication statusPublished - May 2008
MoE publication typeA1 Journal article-refereed

    Research areas

  • alternative oxidase, antimycin A, concanamycin A, mitochondriotoxic, potato scab, spermatozoan motility, Streptomyces scabies, Streptomyces turgidiscabies, thaxtomin A, COMMON SCAB, PHYLOGENETIC ANALYSIS, RESPIRATORY-CHAIN, PATHOGENS, GENE, GRISEOVIRIDIS, CYTOCHROME, COMPONENTS, INHIBITORS, BIOASSAY

ID: 7090893