Anticancer therapeutic efficacy of biogenic Am-ZnO nanoparticles on 2D and 3D tumor models

S. C. Chabattula; P. K. Gupta; S. K. Tripathi; R. Gahtori; P. Padhi; S. Mahapatra; B. K. Biswal; S. K. Singh; K. Dua; J. Ruokolainen; Y. K. Mishra; N. K. Jha; D. K. Bishi; K. K. Kesari

doi:10.1016/j.mtchem.2021.100618

Anticancer therapeutic efficacy of biogenic Am-ZnO nanoparticles on 2D and 3D tumor models

S. C. Chabattula, P. K. Gupta, S. K. Tripathi, R. Gahtori, P. Padhi, S. Mahapatra, B. K. Biswal, S. K. Singh, K. Dua, J. Ruokolainen, Y. K. Mishra, N. K. Jha, D. K. Bishi, K. K. Kesari

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Abstract

The present study fabricates biogenic zinc oxide nanoparticles (ZnO NPs) with the aqueous leaf extract of Annona muricata (Am) plant collected from semi-evergreen forests of Odisha, India. The synthesized Am-ZnO NPs were physicochemically characterized. The ultraviolet/visible spectrum showed the maximum optical absorbance of Am-ZnO NPs at 355 nm. High-resolution transmission electron microscopy analysis presented the nearly spherical shape of Am-ZnO NPs with an average particle size of 80 nm. The net surface charge and hydrodynamic size of Am-ZnO NPs were measured to be ∼−2.59 mV and ∼417 nm, respectively. Am-ZnO NPs were found to be biocompatible and hemocompatible nature. Furthermore, Am-ZnO NPs displayed strong anticancer effects on both 2D and 3D tumor models. We observed a dose-dependent toxicity on both A549 and MOLT4 cells and observed a size reduction in the A549 tumor spheroids. Subsequently, we observed a depolarization in mitochondrial membrane potential of Am-ZnO NP–treated cancer cells leading to the apoptosis induction in cancer cells.

Original language	English
Article number	100618
Number of pages	12
Journal	Materials Today Chemistry
Volume	22
DOIs	https://doi.org/10.1016/j.mtchem.2021.100618
Publication status	Published - Dec 2021
MoE publication type	A1 Journal article-refereed

Keywords

ZnO
Nanoparticles
Anti-cancer
Apoptosis
Leaf's extract
Annona muricata
ZINC-OXIDE NANOPARTICLES
GREEN SYNTHESIS
LEAF EXTRACT
METAL
BIOSYNTHESIS
ANTIOXIDANT
BACTERIA
DELIVERY

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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Anticancer therapeutic efficacy of biogenic Am-ZnO nanoparticles on 2D and 3D tumor modelsFinal published version, 3.24 MBLicence: CC BY

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Chabattula, S. C., Gupta, P. K., Tripathi, S. K., Gahtori, R., Padhi, P., Mahapatra, S., Biswal, B. K., Singh, S. K., Dua, K., Ruokolainen, J., Mishra, Y. K., Jha, N. K., Bishi, D. K., & Kesari, K. K. (2021). Anticancer therapeutic efficacy of biogenic Am-ZnO nanoparticles on 2D and 3D tumor models. Materials Today Chemistry, 22, Article 100618. https://doi.org/10.1016/j.mtchem.2021.100618

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title = "Anticancer therapeutic efficacy of biogenic Am-ZnO nanoparticles on 2D and 3D tumor models",

abstract = "The present study fabricates biogenic zinc oxide nanoparticles (ZnO NPs) with the aqueous leaf extract of Annona muricata (Am) plant collected from semi-evergreen forests of Odisha, India. The synthesized Am-ZnO NPs were physicochemically characterized. The ultraviolet/visible spectrum showed the maximum optical absorbance of Am-ZnO NPs at 355 nm. High-resolution transmission electron microscopy analysis presented the nearly spherical shape of Am-ZnO NPs with an average particle size of 80 nm. The net surface charge and hydrodynamic size of Am-ZnO NPs were measured to be ∼−2.59 mV and ∼417 nm, respectively. Am-ZnO NPs were found to be biocompatible and hemocompatible nature. Furthermore, Am-ZnO NPs displayed strong anticancer effects on both 2D and 3D tumor models. We observed a dose-dependent toxicity on both A549 and MOLT4 cells and observed a size reduction in the A549 tumor spheroids. Subsequently, we observed a depolarization in mitochondrial membrane potential of Am-ZnO NP–treated cancer cells leading to the apoptosis induction in cancer cells.",

keywords = "ZnO, Nanoparticles, Anti-cancer, Apoptosis, Leaf's extract, Annona muricata, ZINC-OXIDE NANOPARTICLES, GREEN SYNTHESIS, LEAF EXTRACT, METAL, BIOSYNTHESIS, ANTIOXIDANT, BACTERIA, DELIVERY",

author = "Chabattula, {S. C.} and Gupta, {P. K.} and Tripathi, {S. K.} and R. Gahtori and P. Padhi and S. Mahapatra and Biswal, {B. K.} and Singh, {S. K.} and K. Dua and J. Ruokolainen and Mishra, {Y. K.} and Jha, {N. K.} and Bishi, {D. K.} and Kesari, {K. K.}",

year = "2021",

month = dec,

doi = "10.1016/j.mtchem.2021.100618",

language = "English",

volume = "22",

journal = "Materials Today Chemistry",

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TY - JOUR

T1 - Anticancer therapeutic efficacy of biogenic Am-ZnO nanoparticles on 2D and 3D tumor models

AU - Chabattula, S. C.

AU - Gupta, P. K.

AU - Tripathi, S. K.

AU - Gahtori, R.

AU - Padhi, P.

AU - Mahapatra, S.

AU - Biswal, B. K.

AU - Singh, S. K.

AU - Dua, K.

AU - Ruokolainen, J.

AU - Mishra, Y. K.

AU - Jha, N. K.

AU - Bishi, D. K.

AU - Kesari, K. K.

PY - 2021/12

Y1 - 2021/12

N2 - The present study fabricates biogenic zinc oxide nanoparticles (ZnO NPs) with the aqueous leaf extract of Annona muricata (Am) plant collected from semi-evergreen forests of Odisha, India. The synthesized Am-ZnO NPs were physicochemically characterized. The ultraviolet/visible spectrum showed the maximum optical absorbance of Am-ZnO NPs at 355 nm. High-resolution transmission electron microscopy analysis presented the nearly spherical shape of Am-ZnO NPs with an average particle size of 80 nm. The net surface charge and hydrodynamic size of Am-ZnO NPs were measured to be ∼−2.59 mV and ∼417 nm, respectively. Am-ZnO NPs were found to be biocompatible and hemocompatible nature. Furthermore, Am-ZnO NPs displayed strong anticancer effects on both 2D and 3D tumor models. We observed a dose-dependent toxicity on both A549 and MOLT4 cells and observed a size reduction in the A549 tumor spheroids. Subsequently, we observed a depolarization in mitochondrial membrane potential of Am-ZnO NP–treated cancer cells leading to the apoptosis induction in cancer cells.

AB - The present study fabricates biogenic zinc oxide nanoparticles (ZnO NPs) with the aqueous leaf extract of Annona muricata (Am) plant collected from semi-evergreen forests of Odisha, India. The synthesized Am-ZnO NPs were physicochemically characterized. The ultraviolet/visible spectrum showed the maximum optical absorbance of Am-ZnO NPs at 355 nm. High-resolution transmission electron microscopy analysis presented the nearly spherical shape of Am-ZnO NPs with an average particle size of 80 nm. The net surface charge and hydrodynamic size of Am-ZnO NPs were measured to be ∼−2.59 mV and ∼417 nm, respectively. Am-ZnO NPs were found to be biocompatible and hemocompatible nature. Furthermore, Am-ZnO NPs displayed strong anticancer effects on both 2D and 3D tumor models. We observed a dose-dependent toxicity on both A549 and MOLT4 cells and observed a size reduction in the A549 tumor spheroids. Subsequently, we observed a depolarization in mitochondrial membrane potential of Am-ZnO NP–treated cancer cells leading to the apoptosis induction in cancer cells.

KW - ZnO

KW - Nanoparticles

KW - Anti-cancer

KW - Apoptosis

KW - Leaf's extract

KW - Annona muricata

KW - ZINC-OXIDE NANOPARTICLES

KW - GREEN SYNTHESIS

KW - LEAF EXTRACT

KW - METAL

KW - BIOSYNTHESIS

KW - ANTIOXIDANT

KW - BACTERIA

KW - DELIVERY

UR - http://www.scopus.com/inward/record.url?scp=85122687265&partnerID=8YFLogxK

U2 - 10.1016/j.mtchem.2021.100618

DO - 10.1016/j.mtchem.2021.100618

M3 - Article

SN - 2468-5194

VL - 22

JO - Materials Today Chemistry

JF - Materials Today Chemistry

M1 - 100618

ER -

Anticancer therapeutic efficacy of biogenic Am-ZnO nanoparticles on 2D and 3D tumor models

Abstract

Keywords

UN SDGs

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