In the course of investigations on brassinosteroid (BS) biosynthetic and metabolic pathways in plants, an approach using HPLC/APCI-MS analysis of underivatized BS for their identification in plant material has been developed. Its application to root-callus suspension cultures of Arabidopsis thaliana led to the first identification of 3-epibrassinolide as natural brassinosteroid.
|Number of pages||6|
|Journal||Collection of Czechoslovak Chemical Communications|
|Publication status||Published - 1 Jan 2001|
|MoE publication type||A1 Journal article-refereed|
- Arabidopsis thaliana
- Isotopic labelling
- Mass spectrometry