Analysis of underivatized brassinosteroids by HPLC/APCI-MS. Occurrence of 3-epibrassinolide in Arabidopsis thaliana

Olga V. Konstantinova; Andrey P. Antonchick; Neil J. Oldham; Vladimir N. Zhabinskii; Vladimir A. Khripach; Bernd Schneider

doi:10.1135/cccc20011729

Analysis of underivatized brassinosteroids by HPLC/APCI-MS. Occurrence of 3-epibrassinolide in Arabidopsis thaliana

Olga V. Konstantinova, Andrey P. Antonchick, Neil J. Oldham, Vladimir N. Zhabinskii, Vladimir A. Khripach, Bernd Schneider^*

^*Corresponding author for this work

Not published at Aalto University

Research output: Contribution to journal › Article › Scientific › peer-review

19 Citations (Scopus)

Abstract

In the course of investigations on brassinosteroid (BS) biosynthetic and metabolic pathways in plants, an approach using HPLC/APCI-MS analysis of underivatized BS for their identification in plant material has been developed. Its application to root-callus suspension cultures of Arabidopsis thaliana led to the first identification of 3-epibrassinolide as natural brassinosteroid.

Original language	English
Pages (from-to)	1729-1734
Number of pages	6
Journal	Collection of Czechoslovak Chemical Communications
Volume	66
Issue number	12
DOIs	https://doi.org/10.1135/cccc20011729
Publication status	Published - 1 Jan 2001
MoE publication type	A1 Journal article-refereed

Keywords

Arabidopsis thaliana
Brassinosteroids
HPLC/APCI-MS
Isotopic labelling
Mass spectrometry
Metabolism
Steroids

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10.1135/cccc20011729

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title = "Analysis of underivatized brassinosteroids by HPLC/APCI-MS. Occurrence of 3-epibrassinolide in Arabidopsis thaliana",

abstract = "In the course of investigations on brassinosteroid (BS) biosynthetic and metabolic pathways in plants, an approach using HPLC/APCI-MS analysis of underivatized BS for their identification in plant material has been developed. Its application to root-callus suspension cultures of Arabidopsis thaliana led to the first identification of 3-epibrassinolide as natural brassinosteroid.",

keywords = "Arabidopsis thaliana, Brassinosteroids, HPLC/APCI-MS, Isotopic labelling, Mass spectrometry, Metabolism, Steroids",

author = "Konstantinova, {Olga V.} and Antonchick, {Andrey P.} and Oldham, {Neil J.} and Zhabinskii, {Vladimir N.} and Khripach, {Vladimir A.} and Bernd Schneider",

year = "2001",

month = jan,

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doi = "10.1135/cccc20011729",

language = "English",

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journal = "Collection of Czechoslovak Chemical Communications",

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Analysis of underivatized brassinosteroids by HPLC/APCI-MS. Occurrence of 3-epibrassinolide in Arabidopsis thaliana. / Konstantinova, Olga V.; Antonchick, Andrey P.; Oldham, Neil J. et al.
In: Collection of Czechoslovak Chemical Communications, Vol. 66, No. 12, 01.01.2001, p. 1729-1734.

Research output: Contribution to journal › Article › Scientific › peer-review

TY - JOUR

T1 - Analysis of underivatized brassinosteroids by HPLC/APCI-MS. Occurrence of 3-epibrassinolide in Arabidopsis thaliana

AU - Konstantinova, Olga V.

AU - Antonchick, Andrey P.

AU - Oldham, Neil J.

AU - Zhabinskii, Vladimir N.

AU - Khripach, Vladimir A.

AU - Schneider, Bernd

PY - 2001/1/1

Y1 - 2001/1/1

N2 - In the course of investigations on brassinosteroid (BS) biosynthetic and metabolic pathways in plants, an approach using HPLC/APCI-MS analysis of underivatized BS for their identification in plant material has been developed. Its application to root-callus suspension cultures of Arabidopsis thaliana led to the first identification of 3-epibrassinolide as natural brassinosteroid.

AB - In the course of investigations on brassinosteroid (BS) biosynthetic and metabolic pathways in plants, an approach using HPLC/APCI-MS analysis of underivatized BS for their identification in plant material has been developed. Its application to root-callus suspension cultures of Arabidopsis thaliana led to the first identification of 3-epibrassinolide as natural brassinosteroid.

KW - Arabidopsis thaliana

KW - Brassinosteroids

KW - HPLC/APCI-MS

KW - Isotopic labelling

KW - Mass spectrometry

KW - Metabolism

KW - Steroids

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U2 - 10.1135/cccc20011729

DO - 10.1135/cccc20011729

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EP - 1734

JO - Collection of Czechoslovak Chemical Communications

JF - Collection of Czechoslovak Chemical Communications

IS - 12

ER -

Analysis of underivatized brassinosteroids by HPLC/APCI-MS. Occurrence of 3-epibrassinolide in Arabidopsis thaliana

Abstract

Keywords

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