Analysis of underivatized brassinosteroids by HPLC/APCI-MS. Occurrence of 3-epibrassinolide in Arabidopsis thaliana

Olga V. Konstantinova, Andrey P. Antonchick, Neil J. Oldham, Vladimir N. Zhabinskii, Vladimir A. Khripach, Bernd Schneider*

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

19 Citations (Scopus)

Abstract

In the course of investigations on brassinosteroid (BS) biosynthetic and metabolic pathways in plants, an approach using HPLC/APCI-MS analysis of underivatized BS for their identification in plant material has been developed. Its application to root-callus suspension cultures of Arabidopsis thaliana led to the first identification of 3-epibrassinolide as natural brassinosteroid.

Original languageEnglish
Pages (from-to)1729-1734
Number of pages6
JournalCollection of Czechoslovak Chemical Communications
Volume66
Issue number12
DOIs
Publication statusPublished - 1 Jan 2001
MoE publication typeA1 Journal article-refereed

Keywords

  • Arabidopsis thaliana
  • Brassinosteroids
  • HPLC/APCI-MS
  • Isotopic labelling
  • Mass spectrometry
  • Metabolism
  • Steroids

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