An Arabidopsis Stomatin-Like Protein Affects Mitochondrial Respiratory Supercomplex Organization

Bernadette Gehl, Chun Pong Lee, Pedro Bota, Michael R. Blatt, Lee J. Sweetlove*

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

Abstract

Stomatins belong to the band-7 protein family, a diverse group of conserved eukaryotic and prokaryoticmembrane proteins involved in the formation of large protein complexes as protein-lipid scaffolds. The Arabidopsis (Arabidopsis thaliana) genome contains two paralogous genes encoding stomatin-like proteins (SLPs; AtSLP1 and AtSLP2) that are phylogenetically related to human SLP2, a protein involved in mitochondrial fusion and protein complex formation in the mitochondrial inner membrane. We used reverse genetics in combination with biochemical methods to investigate the function of AtSLPs. We demonstrate that both SLPs localize to mitochondrial membranes. SLP1 migrates as a large (approximately 3 MDa) complex in blue-native gel electrophoresis. Remarkably, slp1 knockout mutants have reduced protein and activity levels of complex I and supercomplexes, indicating that SLP affects the assembly and/or stability of these complexes. These findings point to a role for SLP1 in the organization of respiratory supercomplexes in Arabidopsis.

Original languageEnglish
Pages (from-to)1389-1400
Number of pages12
JournalPLANT PHYSIOLOGY
Volume164
Issue number3
DOIs
Publication statusPublished - Mar 2014
MoE publication typeA1 Journal article-refereed

Keywords

  • PLANT-MITOCHONDRIA
  • COMPLEX I
  • GENE-EXPRESSION
  • SPFH DOMAIN
  • THALIANA
  • MEMBRANE
  • CHAIN
  • PROHIBITINS
  • RESPONSES
  • STRESS

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