Single-chain antibodies consist of the variable, antigen-binding domains of antibodies joined to a continuous polypeptide by genetically engineered peptide linkers. We have used the flexible interdomain linker region of a fungal cellulase to link together the variable domains of an anti-2-phenyloxazolone IgG1 and show here that the resulting single-chain antibody is efficiently secreted and released to the culture medium of Escherichia coli. The yield of affinity-purified single-chain antibody is 1-2 mg/l of culture medium and its affinity and stability are comparable to those of the corresponding native IgG.
|Number of pages||5|
|Publication status||Published - Oct 1991|
|MoE publication type||A1 Journal article-refereed|
- CELLULASE LINKER PEPTIDE
- EXTRACELLULAR PRODUCTION
- SINGLE-CHAIN ANTIBODY
- VARIABLE DOMAINS