A supramolecular host-guest complex for heparin binding and sensing

Salla Välimäki, Ngong Kodiah Beyeh, Veikko Linko, Robin H.A. Ras, Mauri A. Kostiainen*

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

10 Citations (Scopus)
92 Downloads (Pure)


Heparin is an anionic polysaccharide widely used in clinics as an anticoagulant. However, heparin usage requires an antidote and sensors for safe operation during and after surgeries. In this study, a host-guest complex capable of selective heparin binding and sensing is presented. Heparin binding affinity was studied in solution with a variety of polycationic macrocyclic hosts, a pillar[5]arene and multiple resorcin[4]arenes, by dynamic light scattering, dye displacement assay, isothermal titration calorimetry, and anti-Xa assay. The measurements reveal the significant importance of multivalency in electrostatic host- heparin binding in competitive, application-relevant media. Additionally, to monitor the heparin concentration, a host-guest indicator displacement assay was performed by following the free and bound state of the methyl orange dye in UV-Vis spectroscopic experiments. Furthermore, this colorimetric sensing based on the tertiary host-guest-heparin supramolecular assembly was utilized in the construction of a calibration curve in a range of blood plasma concentrations.

Original languageEnglish
Pages (from-to)14022-14030
Number of pages9
Issue number29
Publication statusPublished - 7 Aug 2018
MoE publication typeA1 Journal article-refereed

Fingerprint Dive into the research topics of 'A supramolecular host-guest complex for heparin binding and sensing'. Together they form a unique fingerprint.

  • Projects

    SYNVIRO: Synthetic Virology Toolbox for the Encapsulation of Therapeutic Polyelectrolytes (SYNVIRO)

    Julin, S., Kostiainen, M., Liu, Q., Välimäki, S., Korpi, A., Ahmed, A. & Anaya Plaza, E.


    Project: Academy of Finland: Other research funding

    HYBER: The Academy of Finland's Centre of Excellence in Molecular Engineering of Biosynthetic Hybrid Materials research (2014-2019)

    Cherian, T., Nonappa, N., Ikkala, O., Bertula, K., Poutanen, M., Morits, M., Hynninen, V., Myllymäki, T., Toivonen, M., Sohrabi, F., Haataja, J., Sanchez Sanchez, A. & Rissanen, S.


    Project: Academy of Finland: Other research funding

    Rationally designed molecular devices through nucleic acid nanotechnology

    Linko, V.


    Project: Academy of Finland: Other research funding


  • Cite this