A self-assembling fluorescent dipeptide conjugate for cell labelling

Steven Kirkham, Ian W. Hamley*, Andrew M. Smith, Ricardo M. Gouveia, Che J. Connon, Mehedi Reza, Janne Ruokolainen

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

12 Citations (Scopus)
316 Downloads (Pure)

Abstract

Derivatives of fluorophore FITC (fluorescein isothiocyanate) are widely used in bioassays to label proteins and cells. An N-terminal leucine dipeptide is attached to FITC, and we show that this simple conjugate molecule is cytocompatible and is uptaken by cells (human dermal and corneal fibroblasts) in contrast to FITC itself. Co-localisation shows that FITC-LL segregates in pen-nuclear and intracellular vesicle regions. Above a critical aggregation concentration, the conjugate is shown to self-assemble into beta-sheet nanostructures comprising molecular bilayers. (C) 2015 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.orgflicenses/by/4.0/).

Original languageEnglish
Pages (from-to)104-108
Number of pages5
JournalColloids and Surfaces B: Biointerfaces
Volume137
Issue number1 Jan 2016
DOIs
Publication statusPublished - 1 Jan 2016
MoE publication typeA1 Journal article-refereed

Keywords

  • Peptides
  • Peptide conjugates
  • Self-assembly
  • Fluorescence
  • SUPRAMOLECULAR HYDROGELS
  • ENZYMATIC FORMATION
  • SMALL MOLECULES
  • PEPTIDE
  • NANOPARTICLES
  • NANOFIBERS/HYDROGELS
  • CYTOTOXICITY
  • DERIVATIVES
  • DELIVERY
  • ENHANCE

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