A self-assembling fluorescent dipeptide conjugate for cell labelling

Steven Kirkham; Ian W. Hamley; Andrew M. Smith; Ricardo M. Gouveia; Che J. Connon; Mehedi Reza; Janne Ruokolainen

doi:10.1016/j.colsurfb.2015.04.062

A self-assembling fluorescent dipeptide conjugate for cell labelling

Steven Kirkham, Ian W. Hamley^*, Andrew M. Smith, Ricardo M. Gouveia, Che J. Connon, Mehedi Reza, Janne Ruokolainen

^*Corresponding author for this work

Research output: Contribution to journal › Article › Scientific › peer-review

12 Citations (Scopus)

316 Downloads (Pure)

Abstract

Derivatives of fluorophore FITC (fluorescein isothiocyanate) are widely used in bioassays to label proteins and cells. An N-terminal leucine dipeptide is attached to FITC, and we show that this simple conjugate molecule is cytocompatible and is uptaken by cells (human dermal and corneal fibroblasts) in contrast to FITC itself. Co-localisation shows that FITC-LL segregates in pen-nuclear and intracellular vesicle regions. Above a critical aggregation concentration, the conjugate is shown to self-assemble into beta-sheet nanostructures comprising molecular bilayers. (C) 2015 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.orgflicenses/by/4.0/).

Original language	English
Pages (from-to)	104-108
Number of pages	5
Journal	Colloids and Surfaces B: Biointerfaces
Volume	137
Issue number	1 Jan 2016
DOIs	https://doi.org/10.1016/j.colsurfb.2015.04.062
Publication status	Published - 1 Jan 2016
MoE publication type	A1 Journal article-refereed

Keywords

Peptides
Peptide conjugates
Self-assembly
Fluorescence
SUPRAMOLECULAR HYDROGELS
ENZYMATIC FORMATION
SMALL MOLECULES
PEPTIDE
NANOPARTICLES
NANOFIBERS/HYDROGELS
CYTOTOXICITY
DERIVATIVES
DELIVERY
ENHANCE

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10.1016/j.colsurfb.2015.04.062

1-s2.0-S0927776515002817-mainFinal published version, 1.16 MBLicence: CC BY

http://www.sciencedirect.com/science/article/pii/S0927776515002817

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Janne Ruokolainen (Manager)
Department of Applied Physics
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Cite this

@article{2ec9ec4c5cab42af8978e057dbf0cf3e,

title = "A self-assembling fluorescent dipeptide conjugate for cell labelling",

abstract = "Derivatives of fluorophore FITC (fluorescein isothiocyanate) are widely used in bioassays to label proteins and cells. An N-terminal leucine dipeptide is attached to FITC, and we show that this simple conjugate molecule is cytocompatible and is uptaken by cells (human dermal and corneal fibroblasts) in contrast to FITC itself. Co-localisation shows that FITC-LL segregates in pen-nuclear and intracellular vesicle regions. Above a critical aggregation concentration, the conjugate is shown to self-assemble into beta-sheet nanostructures comprising molecular bilayers. (C) 2015 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.orgflicenses/by/4.0/).",

keywords = "Peptides, Peptide conjugates, Self-assembly, Fluorescence, SUPRAMOLECULAR HYDROGELS, ENZYMATIC FORMATION, SMALL MOLECULES, PEPTIDE, NANOPARTICLES, NANOFIBERS/HYDROGELS, CYTOTOXICITY, DERIVATIVES, DELIVERY, ENHANCE",

author = "Steven Kirkham and Hamley, {Ian W.} and Smith, {Andrew M.} and Gouveia, {Ricardo M.} and Connon, {Che J.} and Mehedi Reza and Janne Ruokolainen",

year = "2016",

month = jan,

day = "1",

doi = "10.1016/j.colsurfb.2015.04.062",

language = "English",

volume = "137",

pages = "104--108",

journal = "Colloids and surfaces, B: Biointerfaces",

issn = "0927-7765",

publisher = "Elsevier Science B.V.",

number = "1 Jan 2016",

}

A self-assembling fluorescent dipeptide conjugate for cell labelling. / Kirkham, Steven; Hamley, Ian W.; Smith, Andrew M.; Gouveia, Ricardo M.; Connon, Che J.; Reza, Mehedi; Ruokolainen, Janne.

In: Colloids and Surfaces B: Biointerfaces, Vol. 137, No. 1 Jan 2016, 01.01.2016, p. 104-108.

Research output: Contribution to journal › Article › Scientific › peer-review

TY - JOUR

T1 - A self-assembling fluorescent dipeptide conjugate for cell labelling

AU - Kirkham, Steven

AU - Hamley, Ian W.

AU - Smith, Andrew M.

AU - Gouveia, Ricardo M.

AU - Connon, Che J.

AU - Reza, Mehedi

AU - Ruokolainen, Janne

PY - 2016/1/1

Y1 - 2016/1/1

N2 - Derivatives of fluorophore FITC (fluorescein isothiocyanate) are widely used in bioassays to label proteins and cells. An N-terminal leucine dipeptide is attached to FITC, and we show that this simple conjugate molecule is cytocompatible and is uptaken by cells (human dermal and corneal fibroblasts) in contrast to FITC itself. Co-localisation shows that FITC-LL segregates in pen-nuclear and intracellular vesicle regions. Above a critical aggregation concentration, the conjugate is shown to self-assemble into beta-sheet nanostructures comprising molecular bilayers. (C) 2015 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.orgflicenses/by/4.0/).

AB - Derivatives of fluorophore FITC (fluorescein isothiocyanate) are widely used in bioassays to label proteins and cells. An N-terminal leucine dipeptide is attached to FITC, and we show that this simple conjugate molecule is cytocompatible and is uptaken by cells (human dermal and corneal fibroblasts) in contrast to FITC itself. Co-localisation shows that FITC-LL segregates in pen-nuclear and intracellular vesicle regions. Above a critical aggregation concentration, the conjugate is shown to self-assemble into beta-sheet nanostructures comprising molecular bilayers. (C) 2015 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.orgflicenses/by/4.0/).

KW - Peptides

KW - Peptide conjugates

KW - Self-assembly

KW - Fluorescence

KW - SUPRAMOLECULAR HYDROGELS

KW - ENZYMATIC FORMATION

KW - SMALL MOLECULES

KW - PEPTIDE

KW - NANOPARTICLES

KW - NANOFIBERS/HYDROGELS

KW - CYTOTOXICITY

KW - DERIVATIVES

KW - DELIVERY

KW - ENHANCE

UR - http://www.sciencedirect.com/science/article/pii/S0927776515002817

U2 - 10.1016/j.colsurfb.2015.04.062

DO - 10.1016/j.colsurfb.2015.04.062

M3 - Article

VL - 137

SP - 104

EP - 108

JO - Colloids and surfaces, B: Biointerfaces

JF - Colloids and surfaces, B: Biointerfaces

SN - 0927-7765

IS - 1 Jan 2016

ER -

A self-assembling fluorescent dipeptide conjugate for cell labelling

Abstract

Keywords

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