A Novel Method for Mouse Retinal Temperature Determination Based on ERG Photoresponses

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A Novel Method for Mouse Retinal Temperature Determination Based on ERG Photoresponses. / Pitkänen, Marja; Kaikkonen, Ossi; Koskelainen, Ari.

In: Annals of Biomedical Engineering, Vol. 45, No. 10, 2017, p. 2360–2372.

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@article{67a3ee92da5b4e3d87f1345d76724285,
title = "A Novel Method for Mouse Retinal Temperature Determination Based on ERG Photoresponses",
abstract = "This study introduces a novel retinal temperature determination method based on the temperature dependent properties of photoresponses recorded by electroretinography (ERG). The kinetics and amplitudes of ERG photoresponses depend on retinal temperature. Additionally, raising retinal temperature increases the probability of long-wavelength photon absorption, which manifests as temperature dependence of photoreceptor sensitivity. In this study we extract a number of features that represent these properties from the a- and b-waves of mouse ex vivo ERG flash responses and construct three multivariable regression models between temperature and the selected features. The performance of these models was evaluated against a separate test dataset and for two of the models, an RMS temperature determination error of less than 0.50 °C could be reached. Our results demonstrate that the method can be successfully used for reliable retinal temperature determination ex vivo. The method, reflecting the temperature of distal retina, can be applied also in the estimation of retinal pigment epithelium temperature.",
keywords = "Electroretinography, Kinetics, Retina, Retinal pigment epithelium, Temperature-dependence",
author = "Marja Pitk{\"a}nen and Ossi Kaikkonen and Ari Koskelainen",
year = "2017",
doi = "10.1007/s10439-017-1872-y",
language = "English",
volume = "45",
pages = "2360–2372",
journal = "Annals of Biomedical Engineering",
issn = "0090-6964",
number = "10",

}

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TY - JOUR

T1 - A Novel Method for Mouse Retinal Temperature Determination Based on ERG Photoresponses

AU - Pitkänen, Marja

AU - Kaikkonen, Ossi

AU - Koskelainen, Ari

PY - 2017

Y1 - 2017

N2 - This study introduces a novel retinal temperature determination method based on the temperature dependent properties of photoresponses recorded by electroretinography (ERG). The kinetics and amplitudes of ERG photoresponses depend on retinal temperature. Additionally, raising retinal temperature increases the probability of long-wavelength photon absorption, which manifests as temperature dependence of photoreceptor sensitivity. In this study we extract a number of features that represent these properties from the a- and b-waves of mouse ex vivo ERG flash responses and construct three multivariable regression models between temperature and the selected features. The performance of these models was evaluated against a separate test dataset and for two of the models, an RMS temperature determination error of less than 0.50 °C could be reached. Our results demonstrate that the method can be successfully used for reliable retinal temperature determination ex vivo. The method, reflecting the temperature of distal retina, can be applied also in the estimation of retinal pigment epithelium temperature.

AB - This study introduces a novel retinal temperature determination method based on the temperature dependent properties of photoresponses recorded by electroretinography (ERG). The kinetics and amplitudes of ERG photoresponses depend on retinal temperature. Additionally, raising retinal temperature increases the probability of long-wavelength photon absorption, which manifests as temperature dependence of photoreceptor sensitivity. In this study we extract a number of features that represent these properties from the a- and b-waves of mouse ex vivo ERG flash responses and construct three multivariable regression models between temperature and the selected features. The performance of these models was evaluated against a separate test dataset and for two of the models, an RMS temperature determination error of less than 0.50 °C could be reached. Our results demonstrate that the method can be successfully used for reliable retinal temperature determination ex vivo. The method, reflecting the temperature of distal retina, can be applied also in the estimation of retinal pigment epithelium temperature.

KW - Electroretinography

KW - Kinetics

KW - Retina

KW - Retinal pigment epithelium

KW - Temperature-dependence

UR - http://www.scopus.com/inward/record.url?scp=85020518001&partnerID=8YFLogxK

U2 - 10.1007/s10439-017-1872-y

DO - 10.1007/s10439-017-1872-y

M3 - Article

AN - SCOPUS:85020518001

VL - 45

SP - 2360

EP - 2372

JO - Annals of Biomedical Engineering

JF - Annals of Biomedical Engineering

SN - 0090-6964

IS - 10

ER -

ID: 14244084