A Novel Method for Mouse Retinal Temperature Determination Based on ERG Photoresponses

Marja Pitkänen, Ossi Kaikkonen, Ari Koskelainen*

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

5 Citations (Scopus)
128 Downloads (Pure)


This study introduces a novel retinal temperature determination method based on the temperature dependent properties of photoresponses recorded by electroretinography (ERG). The kinetics and amplitudes of ERG photoresponses depend on retinal temperature. Additionally, raising retinal temperature increases the probability of long-wavelength photon absorption, which manifests as temperature dependence of photoreceptor sensitivity. In this study we extract a number of features that represent these properties from the a- and b-waves of mouse ex vivo ERG flash responses and construct three multivariable regression models between temperature and the selected features. The performance of these models was evaluated against a separate test dataset and for two of the models, an RMS temperature determination error of less than 0.50 °C could be reached. Our results demonstrate that the method can be successfully used for reliable retinal temperature determination ex vivo. The method, reflecting the temperature of distal retina, can be applied also in the estimation of retinal pigment epithelium temperature.

Original languageEnglish
Pages (from-to)2360–2372
Number of pages13
JournalAnnals of Biomedical Engineering
Issue number10
Publication statusPublished - 2017
MoE publication typeA1 Journal article-refereed


  • Electroretinography
  • Kinetics
  • Retina
  • Retinal pigment epithelium
  • Temperature-dependence


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