Projects per year
Abstract
DNA self-assembly, and in particular DNA origami, has evolved into a reliable workhorse for organizing organic and inorganic materials with nanometer precision and with exactly controlled stoichiometry. To ensure the intended performance of a given DNA structure, it is beneficial to determine its folding temperature, which in turn yields the best possible assembly of all DNA strands. Here, we show that temperature-controlled sample holders and standard fluorescence spectrometers or dynamic light-scattering setups in a static light-scattering configuration allow for monitoring the assembly progress in real time. With this robust label-free technique, we determine the folding and melting temperatures of a set of different DNA origami structures without the need for more tedious protocols. In addition, we use the method to follow digestion of DNA structures in the presence of DNase I and find strikingly different resistances toward enzymatic degradation depending on the structural design of the DNA object.
Original language | English |
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Pages (from-to) | 4800-4809 |
Number of pages | 10 |
Journal | Biophysical Journal |
Volume | 121 |
Issue number | 24 |
DOIs | |
Publication status | Published - 20 Dec 2022 |
MoE publication type | A1 Journal article-refereed |
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Dive into the research topics of 'A label-free light-scattering method to resolve assembly and disassembly of DNA nanostructures'. Together they form a unique fingerprint.Projects
- 1 Finished
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LIBER Kostiainen: Life-like hybrid materials Kostiainen
Kostiainen, M. (Principal investigator), George, L. (Project Member), Luotonen, O. (Project Member), Zhou, Y. (Project Member), Wierzchowiecka, J. (Project Member), Saarinen, S. (Project Member) & D'Amico, C. (Project Member)
01/01/2022 → 31/12/2024
Project: Academy of Finland: Other research funding
Equipment
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Bioeconomy Research Infrastructure
Seppälä, J. (Manager)
School of Chemical EngineeringFacility/equipment: Facility
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OtaNano - Nanomicroscopy Center
Seitsonen, J. (Manager) & Rissanen, A. (Other)
OtaNanoFacility/equipment: Facility