Projects per year
DNA self-assembly, and in particular DNA origami, has evolved into a reliable workhorse for organizing organic and inorganic materials with nanometer precision and with exactly controlled stoichiometry. To ensure the intended performance of a given DNA structure, it is beneficial to determine its folding temperature, which in turn yields the best possible assembly of all DNA strands. Here, we show that temperature-controlled sample holders and standard fluorescence spectrometers or dynamic light-scattering setups in a static light-scattering configuration allow for monitoring the assembly progress in real time. With this robust label-free technique, we determine the folding and melting temperatures of a set of different DNA origami structures without the need for more tedious protocols. In addition, we use the method to follow digestion of DNA structures in the presence of DNase I and find strikingly different resistances toward enzymatic degradation depending on the structural design of the DNA object.
|Number of pages||10|
|Publication status||Published - 20 Dec 2022|
|MoE publication type||A1 Journal article-refereed|
FingerprintDive into the research topics of 'A label-free light-scattering method to resolve assembly and disassembly of DNA nanostructures'. Together they form a unique fingerprint.
- 1 Active
Center of Excellence in Life-Inspired Hybrid Materials
Kostiainen, M. & Luotonen, O.
01/01/2022 → 31/12/2024
Project: Academy of Finland: Other research funding
Bioeconomy Research Infrastructure
Jukka Seppälä (Manager)School of Chemical Engineering
Anna Rissanen (Manager)Aalto University
OtaNano - Nanomicroscopy Center
Jani Seitsonen (Manager)OtaNano
Findings from Aalto University Update Knowledge of Nanostructures (A Label-free Light-scattering Method To Resolve Assembly and Disassembly of Dna Nanostructures)
1 item of Media coverage
Press/Media: Media appearance