A comparative investigation of H2O2-involved pretreatments on lignocellulosic biomass for enzymatic hydrolysis

Maoyuan Luo; Dong Tian; Fei Shen; Jinguang Hu; Yanzong Zhang; Gang Yang; Yongmei Zeng; Shihuai Deng; Yaodong Hu

doi:10.1007/s13399-018-0364-0

A comparative investigation of H₂O₂-involved pretreatments on lignocellulosic biomass for enzymatic hydrolysis

Maoyuan Luo, Dong Tian, Fei Shen^*, Jinguang Hu, Yanzong Zhang, Gang Yang, Yongmei Zeng, Shihuai Deng, Yaodong Hu

^*Corresponding author for this work

Research output: Contribution to journal › Article › Scientific › peer-review

34 Citations (Scopus)

Abstract

An effective pretreatment to improve cellulose accessibility and facilitate glucose release is crucial in a cellulosic ethanol biorefinery. This work comparatively assessed four H₂O₂-involved pretreatments, i.e., concentrated H₃PO₄ plus H₂O₂ (PHP), H₂O₂–CH₃COOH (HPAC), alkaline-H₂O₂ (AHP), and Fenton chemistry (FC), for their pretreatment performances on wheat straw, poplar, and birch biomass. Substrate characteristics before and after pretreatment were assessed using SEM, XRD, and LSCM. The hydrolytic potentials of the pretreated substrates were compared by Simons’ stain and cellulose–glucose conversion assessment. The results showed that acidic H₂O₂-involved pretreatments (PHP and HPAC) were more efficient in biomass delignification compared to AHP and FC. PHP pretreatment is more promising for cellulosic ethanol production due to its corresponding high glucose yield (368.0 mg g⁻¹) after enzymatic hydrolysis.

Original language	English
Pages (from-to)	321-331
Number of pages	11
Journal	Biomass Conversion and Biorefinery
Volume	9
Issue number	2
DOIs	https://doi.org/10.1007/s13399-018-0364-0
Publication status	Published - 1 Jun 2019
MoE publication type	A1 Journal article-refereed

Keywords

Delignification
Enzymatic saccharification
Enzyme accessibility
Hemicellulose removal
Lignocellulose

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/s13399-018-0364-0

Cite this

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title = "A comparative investigation of H2O2-involved pretreatments on lignocellulosic biomass for enzymatic hydrolysis",

abstract = "An effective pretreatment to improve cellulose accessibility and facilitate glucose release is crucial in a cellulosic ethanol biorefinery. This work comparatively assessed four H2O2-involved pretreatments, i.e., concentrated H3PO4 plus H2O2 (PHP), H2O2–CH3COOH (HPAC), alkaline-H2O2 (AHP), and Fenton chemistry (FC), for their pretreatment performances on wheat straw, poplar, and birch biomass. Substrate characteristics before and after pretreatment were assessed using SEM, XRD, and LSCM. The hydrolytic potentials of the pretreated substrates were compared by Simons{\textquoteright} stain and cellulose–glucose conversion assessment. The results showed that acidic H2O2-involved pretreatments (PHP and HPAC) were more efficient in biomass delignification compared to AHP and FC. PHP pretreatment is more promising for cellulosic ethanol production due to its corresponding high glucose yield (368.0 mg g−1) after enzymatic hydrolysis.",

keywords = "Delignification, Enzymatic saccharification, Enzyme accessibility, Hemicellulose removal, Lignocellulose",

author = "Maoyuan Luo and Dong Tian and Fei Shen and Jinguang Hu and Yanzong Zhang and Gang Yang and Yongmei Zeng and Shihuai Deng and Yaodong Hu",

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AU - Luo, Maoyuan

AU - Tian, Dong

AU - Shen, Fei

AU - Hu, Jinguang

AU - Zhang, Yanzong

AU - Yang, Gang

AU - Zeng, Yongmei

AU - Deng, Shihuai

AU - Hu, Yaodong

PY - 2019/6/1

Y1 - 2019/6/1

N2 - An effective pretreatment to improve cellulose accessibility and facilitate glucose release is crucial in a cellulosic ethanol biorefinery. This work comparatively assessed four H2O2-involved pretreatments, i.e., concentrated H3PO4 plus H2O2 (PHP), H2O2–CH3COOH (HPAC), alkaline-H2O2 (AHP), and Fenton chemistry (FC), for their pretreatment performances on wheat straw, poplar, and birch biomass. Substrate characteristics before and after pretreatment were assessed using SEM, XRD, and LSCM. The hydrolytic potentials of the pretreated substrates were compared by Simons’ stain and cellulose–glucose conversion assessment. The results showed that acidic H2O2-involved pretreatments (PHP and HPAC) were more efficient in biomass delignification compared to AHP and FC. PHP pretreatment is more promising for cellulosic ethanol production due to its corresponding high glucose yield (368.0 mg g−1) after enzymatic hydrolysis.

AB - An effective pretreatment to improve cellulose accessibility and facilitate glucose release is crucial in a cellulosic ethanol biorefinery. This work comparatively assessed four H2O2-involved pretreatments, i.e., concentrated H3PO4 plus H2O2 (PHP), H2O2–CH3COOH (HPAC), alkaline-H2O2 (AHP), and Fenton chemistry (FC), for their pretreatment performances on wheat straw, poplar, and birch biomass. Substrate characteristics before and after pretreatment were assessed using SEM, XRD, and LSCM. The hydrolytic potentials of the pretreated substrates were compared by Simons’ stain and cellulose–glucose conversion assessment. The results showed that acidic H2O2-involved pretreatments (PHP and HPAC) were more efficient in biomass delignification compared to AHP and FC. PHP pretreatment is more promising for cellulosic ethanol production due to its corresponding high glucose yield (368.0 mg g−1) after enzymatic hydrolysis.

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