A bioactive hybrid three-dimensional tissue-engineering construct for cartilage repair

Mari Ainola*, Waclaw Tomaszewski, Barbara Ostrowska, Ewa Wesolowska, H. Daniel Wagner, Wojciech Swieszkowski, Tarvo Sillat, Emilia Peltola, Yrjö T. Konttinen

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

13 Citations (Scopus)

Abstract

The aim was to develop a hybrid three-dimensional-tissue engineering construct for chondrogenesis. The hypothesis was that they support chondrogenesis. A biodegradable, highly porous polycaprolactone-grate was produced by solid freeform fabrication. The polycaprolactone support was coated with a chitosan/polyethylene oxide nanofibre sheet produced by electrospinning. Transforming growth factor-β3-induced chondrogenesis was followed using the following markers: sex determining region Y/-box 9, runt-related transcription factor 2 and collagen II and X in quantitative real-time polymerase chain reaction, histology and immunostaining. A polycaprolactone-grate and an optimized chitosan/polyethylene oxide nanofibre sheet supported cellular aggregation, chondrogenesis and matrix formation. In tissue engineering constructs, the sheets were seeded first with mesenchymal stem cells and then piled up according to the lasagne principle. The advantages of such a construct are (1) the cells do not need to migrate to the tissue engineering construct and therefore pore size and interconnectivity problems are omitted and (2) the cell-tight nanofibre sheet and collagen-fibre network mimic a cell culture platform for mesenchymal stem cells/chondrocytes (preventing escape) and hinders in-growth of fibroblasts and fibrous scarring (preventing capture). This allows time for the slowly progressing, multiphase true cartilage regeneration.

Original languageEnglish
Pages (from-to)873-885
Number of pages13
JournalJournal of Biomaterials Applications
Volume30
Issue number6
DOIs
Publication statusPublished - 1 Jan 2016
MoE publication typeA1 Journal article-refereed

Keywords

  • cartilage
  • chitosan
  • chondrogenesis
  • nanofibres
  • rapid prototyping
  • Scaffold

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