3D printing and properties of cellulose nanofibrils-reinforced quince seed mucilage bio-inks

Hossein Bani Asadi; Roberta Teixeira Polez; Erfan Kimiaei; Zahraalsadat Madani; Orlando Rojas Gaona; Monika Österberg; Jukka Seppälä

doi:10.1016/j.ijbiomac.2021.10.078

3D printing and properties of cellulose nanofibrils-reinforced quince seed mucilage bio-inks

Hossein Bani Asadi, Roberta Teixeira Polez, Erfan Kimiaei, Zahraalsadat Madani, Orlando Rojas Gaona, Monika Österberg, Jukka Seppälä^*

^*Corresponding author for this work

University of British Columbia

Research output: Contribution to journal › Article › Scientific › peer-review

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Abstract

Plant-based hydrogels have attracted great attention in biomedical fields since they are biocompatible and based on natural, sustainable, cost-effective, and widely accessible sources. Here, we introduced new viscoelastic bio-inks composed of quince seed mucilage and cellulose nanofibrils (QSM/CNF) easily extruded into 3D lattice structures through direct ink writing in ambient conditions. The QSM/CNF inks enabled precise control on printing fidelity where CNF endowed objects with shape stability after freeze-drying and with suitable porosity, water uptake capacity, and mechanical strength. The compressive and elastic moduli of samples produced at the highest CNF content were both increased by ~100% (from 5.1 ± 0.2 kPa and 32 ± 1 kPa to 10.7 ± 0.5 and 64 ± 2 kPa, respectively). These values ideally matched those reported for soft tissues; accordingly, the cell compatibility of the printed samples was evaluated against HepG2 cells (human liver cancer). The results confirmed the 3D hydrogels as being non-cytotoxic and suitable to support attachment, survival, and proliferation of the cells. All in all, the newly developed inks allowed sustainable 3D bio-hydrogels fitting the requirements as scaffolds for soft tissue engineering.

Original language	English
Pages (from-to)	1098-1107
Number of pages	10
Journal	International Journal of Biological Macromolecules
Volume	192
Early online date	16 Oct 2021
DOIs	https://doi.org/10.1016/j.ijbiomac.2021.10.078
Publication status	Published - 1 Dec 2021
MoE publication type	A1 Journal article-refereed

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10.1016/j.ijbiomac.2021.10.078Licence: CC BY

3D printing and properties of cellulose nanofibrils-reinforced quince seedFinal published version, 4.63 MBLicence: CC BY

BIOECONOMY: BIOECONOMY Alliance for excellence in sustainable biomass refining
Seppälä, J. (Principal investigator)
01/01/2020 → 31/12/2023
Project: Academy of Finland: Other research funding
VALUEBIOMAT: Bio-oils based polymeric composites; value chain from syntheisis to additive manufacturing
Seppälä, J. (Principal investigator), Äkräs, L. (Project Member), Revitzer, H. (Project Member), van Bochove, B. (Project Member), Baniasadi, H. (Project Member), Farzan, A. (Project Member), Dienel, K. (Project Member), Teotia, A. (Project Member), Borandeh, S. (Project Member) & Madani, M. (Project Member)
01/06/2019 → 28/02/2023
Project: Academy of Finland: Strategic research funding
FinnCERES: Competence Center for the Materials Bioeconomy: A Flagship for our Sustainable Future
Mäkelä, K. (Principal investigator)
01/05/2018 → 31/12/2022
Project: Academy of Finland: Other research funding

Bioeconomy Research Infrastructure
Seppälä, J. (Manager)
School of Chemical Engineering
Facility/equipment: Facility

Cite this

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title = "3D printing and properties of cellulose nanofibrils-reinforced quince seed mucilage bio-inks",

abstract = "Plant-based hydrogels have attracted great attention in biomedical fields since they are biocompatible and based on natural, sustainable, cost-effective, and widely accessible sources. Here, we introduced new viscoelastic bio-inks composed of quince seed mucilage and cellulose nanofibrils (QSM/CNF) easily extruded into 3D lattice structures through direct ink writing in ambient conditions. The QSM/CNF inks enabled precise control on printing fidelity where CNF endowed objects with shape stability after freeze-drying and with suitable porosity, water uptake capacity, and mechanical strength. The compressive and elastic moduli of samples produced at the highest CNF content were both increased by ~100% (from 5.1 ± 0.2 kPa and 32 ± 1 kPa to 10.7 ± 0.5 and 64 ± 2 kPa, respectively). These values ideally matched those reported for soft tissues; accordingly, the cell compatibility of the printed samples was evaluated against HepG2 cells (human liver cancer). The results confirmed the 3D hydrogels as being non-cytotoxic and suitable to support attachment, survival, and proliferation of the cells. All in all, the newly developed inks allowed sustainable 3D bio-hydrogels fitting the requirements as scaffolds for soft tissue engineering.",

author = "{Bani Asadi}, Hossein and {Teixeira Polez}, Roberta and Erfan Kimiaei and Zahraalsadat Madani and {Rojas Gaona}, Orlando and Monika {\"O}sterberg and Jukka Sepp{\"a}l{\"a}",

note = "The authors would like to acknowledge the Academy of Finland funding; No. 327248 (ValueBiomat) and 327865 (Bioeconomy). This work was a part of the Academy of Finland's Flagship Programme under Projects No. 318890 and 318891 (Competence Center for Materials Bioeconomy, FinnCERES). The authors would also like to thank Ms. Marja K{\"a}rkk{\"a}inen for providing TEMPO-CNF and the Biohybrid Materials Research Group (Aalto University) for providing the HepG2 cells.",

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AU - Bani Asadi, Hossein

AU - Teixeira Polez, Roberta

AU - Kimiaei, Erfan

AU - Madani, Zahraalsadat

AU - Rojas Gaona, Orlando

AU - Österberg, Monika

AU - Seppälä, Jukka

N1 - The authors would like to acknowledge the Academy of Finland funding; No. 327248 (ValueBiomat) and 327865 (Bioeconomy). This work was a part of the Academy of Finland's Flagship Programme under Projects No. 318890 and 318891 (Competence Center for Materials Bioeconomy, FinnCERES). The authors would also like to thank Ms. Marja Kärkkäinen for providing TEMPO-CNF and the Biohybrid Materials Research Group (Aalto University) for providing the HepG2 cells.

PY - 2021/12/1

Y1 - 2021/12/1

N2 - Plant-based hydrogels have attracted great attention in biomedical fields since they are biocompatible and based on natural, sustainable, cost-effective, and widely accessible sources. Here, we introduced new viscoelastic bio-inks composed of quince seed mucilage and cellulose nanofibrils (QSM/CNF) easily extruded into 3D lattice structures through direct ink writing in ambient conditions. The QSM/CNF inks enabled precise control on printing fidelity where CNF endowed objects with shape stability after freeze-drying and with suitable porosity, water uptake capacity, and mechanical strength. The compressive and elastic moduli of samples produced at the highest CNF content were both increased by ~100% (from 5.1 ± 0.2 kPa and 32 ± 1 kPa to 10.7 ± 0.5 and 64 ± 2 kPa, respectively). These values ideally matched those reported for soft tissues; accordingly, the cell compatibility of the printed samples was evaluated against HepG2 cells (human liver cancer). The results confirmed the 3D hydrogels as being non-cytotoxic and suitable to support attachment, survival, and proliferation of the cells. All in all, the newly developed inks allowed sustainable 3D bio-hydrogels fitting the requirements as scaffolds for soft tissue engineering.

AB - Plant-based hydrogels have attracted great attention in biomedical fields since they are biocompatible and based on natural, sustainable, cost-effective, and widely accessible sources. Here, we introduced new viscoelastic bio-inks composed of quince seed mucilage and cellulose nanofibrils (QSM/CNF) easily extruded into 3D lattice structures through direct ink writing in ambient conditions. The QSM/CNF inks enabled precise control on printing fidelity where CNF endowed objects with shape stability after freeze-drying and with suitable porosity, water uptake capacity, and mechanical strength. The compressive and elastic moduli of samples produced at the highest CNF content were both increased by ~100% (from 5.1 ± 0.2 kPa and 32 ± 1 kPa to 10.7 ± 0.5 and 64 ± 2 kPa, respectively). These values ideally matched those reported for soft tissues; accordingly, the cell compatibility of the printed samples was evaluated against HepG2 cells (human liver cancer). The results confirmed the 3D hydrogels as being non-cytotoxic and suitable to support attachment, survival, and proliferation of the cells. All in all, the newly developed inks allowed sustainable 3D bio-hydrogels fitting the requirements as scaffolds for soft tissue engineering.

U2 - 10.1016/j.ijbiomac.2021.10.078

DO - 10.1016/j.ijbiomac.2021.10.078

M3 - Article

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VL - 192

SP - 1098

EP - 1107

JO - International Journal of Biological Macromolecules

JF - International Journal of Biological Macromolecules

ER -

3D printing and properties of cellulose nanofibrils-reinforced quince seed mucilage bio-inks

Abstract

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Projects

BIOECONOMY: BIOECONOMY Alliance for excellence in sustainable biomass refining

VALUEBIOMAT: Bio-oils based polymeric composites; value chain from syntheisis to additive manufacturing

FinnCERES: Competence Center for the Materials Bioeconomy: A Flagship for our Sustainable Future

Equipment

Bioeconomy Research Infrastructure

Cite this