Systematic approach to study alternative splicing from scRNA-seq in cancer

Project Details


Alternative splicing has been shown to play important roles in cancer development. Currently, most alternative splicing studies stay on bulk RNA-seq level. Although it is estimated that 92% of human genes undergo alternative splicing, studies using bulk RNA-seq can only uncover alternative splicing for a small proportion of genes in cancer. This is due to the heterogeneous nature of cancer, in which significant splicing events specific for a cell population are averaged out over all heterogenous cells. Therefore, it is natural to resort to single cell RNA sequencing (scRNA-seq) to handle the heterogeneity in cancer. In this project, I will use a multi-omics approach to systematically study alternative splicing and its regulation from scRNA-seq of cancer.
Effective start/end date01/09/202028/02/2024

Collaborative partners


Explore the research topics touched on by this project. These labels are generated based on the underlying awards/grants. Together they form a unique fingerprint.
  • Genomic monitoring of SARS-CoV-2 uncovers an Nsp1 deletion variant that modulates type I interferon response

    Lin, J., Tang, C., Wei, H., Du, B., Chen, C., Wang, M., Zhou, Y., Yu, M., Cheng, L., Kuivanen, S., Ogando, N. S., Levanov, L., Zhao, Y., Li, C., Zhou, R., Li, Z., Zhang, Y., Sun, K., Wang, C., Chen, L., & 52 othersXiao, X., Zheng, X., Chen, S., Yang, R., Zhang, D., Xu, M., Song, J., Wang, D., Li, Y., Lei, S., Zeng, W., Yang, Q., He, P., Zhang, Y., Zhou, L., Cao, L., Luo, F., Liu, H., Wang, L., Ye, F., Zhang, M., Li, M., Li, X., Li, K., Ke, B., Xu, J., Yang, H., He, S., Pan, M., Yan, Y., Zha, Y., Jiang, L., Yu, C., Liu, Y., Xu, Z., Li, Q., Jiang, Y., Sun, J., Hong, W., Wei, H., Lu, G., Vapalahti, O., Luo, Y., Wei, Y., Connor, T., Tan, W., Snijder, E. J., Smura, T., Li, W., Geng, J., Ying, B. & Chen, L., 10 Mar 2021, In: Cell Host and Microbe. 29, 3, p. 489-502.e8 22 p.

    Research output: Contribution to journalArticleScientificpeer-review

    Open Access
    79 Citations (Scopus)
    48 Downloads (Pure)