Engineering orthogonal protein-peptide pairs for the production of stronger recombinant spider silk

Project Details


The impressive properties of spider silk and the elegant spinning system of spiders have inspired scientists for decades. The properties of recombinant spider silk are, however, still far from those of the natural material. A major challenge in mimicking spider silk is the production of spider dragline silk proteins in bacteria, as these need to be thousands of amino acids long and highly repetitive. This project aims at solving the problem by producing shorter spider silk protein fragments, conjugating them after purification using protein-peptide conjugation pairs, and pulling fibers from the long silk proteins obtained by conjugation. In order to do so, new conjugation pairs need to be developed. The end product, spider silk spun from native-sized silk proteins, is expected to have dramatically improved mechanical properties compared to fibers prepared from shorter fragments.
Short titleAranko Sesilja
Effective start/end date01/09/201731/10/2021