Data from: Single-cell genomics reveals hundreds of coexisting subpopulations in wild Prochlorococcus

  • Nadav Kashtan (Contributor)
  • Sara E. Roggensack (Contributor)
  • Sébastien Rodrigue (Contributor)
  • Jessie W. Thompson (Contributor)
  • Steven J. Biller (Contributor)
  • Allison Coe (Contributor)
  • Huiming Ding (Contributor)
  • Pekka Marttinen (Creator)
  • Rex R. Malmstrom (Contributor)
  • Roman Stocker (Contributor)
  • MJ Follows (Contributor)
  • R Stepanauskas (Creator)
  • Chisholm SW (Creator)



    Extensive genomic diversity within coexisting members of a microbial species has been revealed through selected cultured isolates and metagenomic assemblies. Yet, the cell-by-cell genomic composition of wild uncultured populations of co-occurring cells is largely unknown. In this work, we applied large-scale single-cell genomics to study populations of the globally abundant marine cyanobacterium Prochlorococcus. We show that they are composed of hundreds of subpopulations with distinct “genomic backbones,” each backbone consisting of a different set of core gene alleles linked to a small distinctive set of flexible genes. These subpopulations are estimated to have diverged at least a few million years ago, suggesting ancient, stable niche partitioning. Such a large set of coexisting subpopulations may be a general feature of free-living bacterial species with huge populations in highly mixed habitats.
    Date made available25 Apr 2014
    PublisherDryad Digital Repository

    Research Output

    Single-Cell Genomics Reveals Hundreds of Coexisting Subpopulations in Wild Prochlorococcus

    Kashtan, N., Roggensack, S. E., Rodrigue, S., Thompson, J. W., Biller, S. J., Coe, A., Ding, H., Marttinen, P., Malmstrom, R. R. & Stocker, R., 2014, In : Science. 344, 6182, p. 416-420

    Research output: Contribution to journalArticleScientificpeer-review

    229 Citations (Scopus)

    Cite this

    Kashtan, N. (Contributor), Roggensack, S. E. (Contributor), Rodrigue, S. (Contributor), Thompson, J. W. (Contributor), Biller, S. J. (Contributor), Coe, A. (Contributor), Ding, H. (Contributor), Marttinen, P. (Creator), Malmstrom, R. R. (Contributor), Stocker, R. (Contributor), Follows, M. (Contributor), Stepanauskas, R. (Creator), SW, C. (Creator) (25 Apr 2014). Data from: Single-cell genomics reveals hundreds of coexisting subpopulations in wild Prochlorococcus. Dryad Digital Repository. 10.5061/dryad.9r0p6